Isobe Tomohiro, Ikebata Yoshihisa, Do Lanh Thi Kim, Tanihara Fuminori, Taniguchi Masayasu, Otoi Takeshige
Laboratory of Animal Reproduction, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
Kagoshima Prefectural Government Livestock Division, Kagoshima Prefecture, Kagoshima, Japan.
Anim Sci J. 2015 Jul;86(7):661-5. doi: 10.1111/asj.12341. Epub 2014 Dec 9.
The optimization of single-embryo culture conditions is very important, particularly in the in vitro production of bovine embryos using the ovum pick-up (OPU) procedure. The purpose of this study was to examine the development of embryos derived from oocytes obtained by OPU that were cultured either individually or in groups in medium supplemented with or without sericin and to investigate the viability of the frozen-thawed embryos after a direct transfer. When two-cell-stage embryos were cultured either individually or in groups for 7 days in CR1aa medium supplemented with or without 0.5% sericin, the rates of development to blastocysts and freezable blastocysts were significantly lower for the embryos cultured individually without sericin than for the embryos cultured in groups with or without sericin. Moreover, the rate of development to freezable blastocysts of the embryos cultured individually with sericin was significantly higher than that of the embryos cultured without sericin. When the frozen-thawed embryos were transferred directly to recipients, the rates of pregnancy, abortion, stillbirth and normal calving in the recipients were similar among the groups, irrespective of the culture conditions and sericin supplementation. Our findings indicate that supplementation with sericin during embryo culture improves the quality of the embryos cultured individually but not the viability of the frozen-thawed embryos after transfer to recipients.
单胚胎培养条件的优化非常重要，尤其是在采用采卵（OPU）程序进行牛胚胎的体外生产过程中。本研究的目的是检测通过OPU获得的卵母细胞所衍生胚胎的发育情况，这些胚胎分别在添加或不添加丝胶蛋白的培养基中单独培养或成组培养，并研究直接移植后冻融胚胎的活力。当二细胞期胚胎在添加或不添加0.5%丝胶蛋白的CR1aa培养基中单独或成组培养7天时，与在添加或不添加丝胶蛋白的培养基中进行成组培养的胚胎相比，在不添加丝胶蛋白的培养基中单独培养的胚胎发育至囊胚和可冷冻囊胚的比率显著更低。此外，在添加丝胶蛋白的培养基中单独培养的胚胎发育至可冷冻囊胚的比率显著高于不添加丝胶蛋白培养的胚胎。当将冻融胚胎直接移植到受体中时，无论培养条件和丝胶蛋白添加情况如何，各实验组受体的妊娠率、流产率、死产率和正常产犊率均相似。我们的研究结果表明，胚胎培养过程中添加丝胶蛋白可提高单独培养胚胎的质量，但对移植到受体后的冻融胚胎活力没有影响。
