Hajarian H, Aghaz F, Karami Shabankareh H
IVF Laboratory, Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran.
IVF Laboratory, Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran; Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Theriogenology. 2017 Apr 1;92:144-148. doi: 10.1016/j.theriogenology.2016.12.027. Epub 2016 Dec 24.
Sericin is a water-soluble component of silk and has been used as a biomaterial due to its antibacterial and ultraviolet radiation-resistant properties. This study was designed to evaluate the effect of sericin supplementation, as a serum replacement, in maturation and culture media on the meiotic competence of oocytes or in vitro culture of ovine embryos. In experiment 1, oocytes were matured in the presence of 10% fetal ovine serum (FOS), 0.1% polyvinyl alcohol (PVA) and different concentrations of sericin (0.1, 0.5, 1 and 2.5%), for 24 h. The addition of 0.5% sericin to maturation medium increased the rates of maturation to metaphase II of oocytes compared with those in cultures with 0.1% PVA. Following fertilization, blastocyst development was higher for oocytes matured with 0.5% of sericin compared with 0.1% PVA. However, the rates of nuclear maturation of oocytes and blastocyst development under FOS and 0.5% sericin were not significantly different. In experiment 2, presumptive zygotes were cultured in the presence of 10% FOS, 0.1% PVA and different concentrations of sericin (0.1, 0.5, 1 and 2.5%), for 7-8 days. The addition of 0.5% sericin to culture medium increased the blastocyst rate compared with those in cultures without sericin or addition of 0.1% PVA and 10% FOS. These results indicate the feasibility of sericin as an alternative protein supplement for IVM and IVC in ovine oocytes and zygotes.
丝胶蛋白是蚕丝的一种水溶性成分，因其抗菌和抗紫外线特性而被用作生物材料。本研究旨在评估作为血清替代品添加到成熟培养液和胚胎培养液中的丝胶蛋白对卵母细胞减数分裂能力或绵羊胚胎体外培养的影响。在实验1中，卵母细胞在含有10%胎羊血清（FOS）、0.1%聚乙烯醇（PVA）和不同浓度丝胶蛋白（0.1%、0.5%、1%和2.5%）的培养液中成熟培养24小时。与含有0.1% PVA的培养液相比，在成熟培养液中添加0.5%丝胶蛋白可提高卵母细胞成熟至中期II的比例。受精后，与0.1% PVA组相比，用0.5%丝胶蛋白成熟的卵母细胞的囊胚发育率更高。然而，FOS和0.5%丝胶蛋白组的卵母细胞核成熟率和囊胚发育率无显著差异。在实验2中，假定受精卵在含有10% FOS、0.1% PVA和不同浓度丝胶蛋白（0.1%、0.5%、1%和2.5%）的培养液中培养7 - 8天。与不添加丝胶蛋白或添加0.1% PVA和10% FOS的培养液相比，在培养液中添加0.5%丝胶蛋白可提高囊胚率。这些结果表明丝胶蛋白作为绵羊卵母细胞和受精卵体外成熟（IVM）和体外培养（IVC）的替代蛋白质补充剂是可行的。
