Ohga S, Yoshikai Y, Matsumoto K, Kishihara K, Matsuzaki G, Nomoto K
Department of Immunology, Kyushu University, Fukuoka, Japan.
Immunology. 1989 Aug;67(4):543-6.
To search the abnormality in prethymic T-cell precursors in lpr/lpr(lpr) mice, rearrangement and expression of T-cell receptor (TcR) genes were investigated in long-term cultured bone marrow (LTBM) cells of lpr mice, in which the developmental steps of T-cell precursors may be better synchronized than those in bone marrow (BM) cells. Neither rearrangment nor expression of TCR gamma and delta genes were detected in the LTBM cells from +/+ control mice, whereas some gamma gene rearrangements were detected in those derived from lpr mice, irrespective of the genetic background. When BM cells or LTBM cells from lpr mice were transplanted into supralethally irradiated +/+ mice the lpr-derived BM cells appeared earlier in the thymus of the recipient mice than +/+-derived BM cells and the recipients suffered from lethal wasting syndrome. In addition, the lpr-derived BM cells showed higher activity in colony-forming unit spleen (CFUs) than the +/+-derived BM cells. These results suggest that the T-cell progenitors in the BM of lpr mice may be different not only in quantity but also in quality from those of +/+ mice.
为了探寻lpr/lpr（lpr）小鼠胸腺前体T细胞前体细胞中的异常情况，研究人员对lpr小鼠长期培养的骨髓（LTBM）细胞中的T细胞受体（TcR）基因重排和表达进行了研究，在LTBM细胞中T细胞前体的发育步骤可能比骨髓（BM）细胞中的更好同步化。在+/+对照小鼠的LTBM细胞中未检测到TCRγ和δ基因的重排或表达，而在源自lpr小鼠的LTBM细胞中检测到了一些γ基因重排，与遗传背景无关。当将lpr小鼠的BM细胞或LTBM细胞移植到经超致死剂量照射的+/+小鼠中时，源自lpr的BM细胞在受体小鼠胸腺中出现的时间比源自+/+的BM细胞更早，且受体小鼠患有致死性消瘦综合征。此外，源自lpr的BM细胞在脾集落形成单位（CFUs）中的活性高于源自+/+的BM细胞。这些结果表明，lpr小鼠骨髓中的T细胞祖细胞不仅在数量上而且在质量上可能与+/+小鼠的不同。
