Andreeva A M
Zh Evol Biokhim Fiziol. 2013 Nov-Dec;49(6):394-402.
In blood and tissue fluid of squamulose redeye, rock perches, starlet, and two species of electric rays, with aid of methods of proteomics, there were identified ependemines, vitellogenins, immunoglobulins, and albumin-like proteins (ALP). For all proteins except for ALP, identification was successful with use of MALDI mass-spectroscopy in the tandem regime. Reconstruction of fragments of amino acid sequences was performed with databases of fish with the sequenced genome and mammals. Problems at the ALP identification are connected with a high level of their structural diversity and the absence of similarity with mammalian proteins.
在叶状红眼睛鱼、岩鲈、星斑川鲽以及两种电鳐的血液和组织液中，借助蛋白质组学方法鉴定出了内啡肽、卵黄蛋白原、免疫球蛋白和类白蛋白蛋白（ALP）。除ALP外，所有蛋白质均通过串联模式的基质辅助激光解吸电离质谱成功鉴定。利用已测序基因组的鱼类和哺乳动物数据库进行了氨基酸序列片段的重建。ALP鉴定的问题与其高度的结构多样性以及与哺乳动物蛋白质缺乏相似性有关。
