Li Gang, Zhou Kaiyue, Zhao Guodong, Qian Heying, Xu Anying
1Jiangsu University of Science and Technology, Zhenjiang, 212018 Jiangsu People's Republic of China.
2Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, 212018 Jiangsu People's Republic of China.
3 Biotech. 2019 Apr;9(4):152. doi: 10.1007/s13205-019-1669-9. Epub 2019 Mar 25.
Novel alternative splicing events were identified from BmNPV-susceptible and -resistant silkworm strains after BmNPV infection using high-throughput RNA-sequencing strategy. In total, 12.82 Gb clean RNA-seq data were generated for the two midgut samples from BmNPV-susceptible and -resistant silkworm strains, and 14.78 Gb clean data for the two fat body samples. The number of alternative splicing events and isoforms in the BmNPV-susceptible silkworm strain was more than that in the BmNPV-resistant silkworm strain. Furthermore, alternative splicing genes uniquely present in BmNPV-resistant silkworm strain were involved in functions about ribosome, whereas, alternative splicing genes uniquely present in BmNPV-susceptible silkworm strain were implicated in functions like DNA helicase activity and signal transduction. Additionally, 33 expressed SR or SR-like proteins were identified, and three genes encoding SR or SR-like proteins ( and ) have a higher number of different alternative splicing events between two silkworm strains. The present study suggested BmNPV treatment may have a smaller effect on the mRNA transcription in BmNPV-resistant silkworms than that in BmNPV-susceptible silkworms, and functions of alternative splicing genes are different between the two silkworm strains.
采用高通量RNA测序策略，在BmNPV感染后的BmNPV敏感和抗性家蚕品系中鉴定出新型可变剪接事件。总共为来自BmNPV敏感和抗性家蚕品系的两个中肠样本生成了12.82Gb的干净RNA-seq数据，为两个脂肪体样本生成了14.78Gb的干净数据。BmNPV敏感家蚕品系中的可变剪接事件和异构体数量多于BmNPV抗性家蚕品系。此外，BmNPV抗性家蚕品系中独特存在的可变剪接基因参与核糖体相关功能，而BmNPV敏感家蚕品系中独特存在的可变剪接基因涉及DNA解旋酶活性和信号转导等功能。此外，鉴定出33种表达的SR或SR样蛋白，并且三个编码SR或SR样蛋白的基因（和）在两个家蚕品系之间具有更多数量的不同可变剪接事件。本研究表明，BmNPV处理对BmNPV抗性家蚕mRNA转录的影响可能小于对BmNPV敏感家蚕的影响，并且两个家蚕品系之间可变剪接基因的功能不同。
