Zhang Jie, Guo Gang, Chen Lei, Li Junfeng, Yuan Xianjun, Yu Chengqun, Shimojo Masataka, Shao Tao
Institute of Ensiling and Processing of Grass, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, China.
Anim Sci J. 2015 Jun;86(6):595-602. doi: 10.1111/asj.12340. Epub 2014 Dec 11.
The objective of this study was to evaluate effects of lactic acid bacteria and propionic acid on the fermentation quality and aerobic stability of oats-common vetch mixed silage by using a small-scale fermentation system on the Tibetan plateau. (i) An inoculant (Lactobacillus plantarum) (L) or (ii) propionic acid (P) or (iii) inoculant + propionic acid (PL) were used as additives. After fermenting for 60 days, silos were opened and the aerobic stability was tested for the following 15 days. The results showed that all silages were well preserved with low pH and NH3 -N, and high lactic acid content and V-scores. L and PL silages showed higher (P < 0.05) lactic acid and crude protein content than the control silage. P silage inhibited lactic acid production. Under aerobic conditions, L silage had similar yeast counts as the control silage (> 10(5) cfu/g fresh matter (FM)); however, it numerically reduced aerobic stability for 6 h. P and PL silages showed fewer yeasts (< 10(5) cfu/g FM) (P < 0.05) and markedly improved the aerobic stability (> 360 h). The result suggested that PL is the best additive as it could not only improved fermentation quality, but also aerobic stability of oats-common vetch mixed silage on the Tibetan plateau.
本研究的目的是通过在青藏高原上使用小规模发酵系统,评估乳酸菌和丙酸对燕麦-紫云英混合青贮饲料发酵品质和好氧稳定性的影响。(i)接种剂(植物乳杆菌)(L)或(ii)丙酸(P)或(iii)接种剂+丙酸(PL)用作添加剂。发酵60天后,打开青贮窖,并在接下来的15天内测试好氧稳定性。结果表明,所有青贮饲料均保存良好,pH值和氨态氮含量低,乳酸含量和V值高。L和PL青贮饲料的乳酸和粗蛋白含量均高于对照青贮饲料(P<0.05)。P青贮饲料抑制乳酸产生。在有氧条件下,L青贮饲料的酵母数量与对照青贮饲料相似(>10⁵ cfu/g鲜物质(FM));然而,其好氧稳定性在数值上降低了6小时。P和PL青贮饲料的酵母数量较少(<10⁵ cfu/g FM)(P<0.05),并显著提高了好氧稳定性(>360小时)。结果表明,PL是最佳添加剂,因为它不仅可以提高青藏高原燕麦-紫云英混合青贮饲料的发酵品质,还可以提高其好氧稳定性。