Wajs Ewelina, Molina-Ontoria Agustín, Nielsen Thorbjørn Terndrup, Echegoyen Luis, Fragoso Alex
Nanobiotechnology & Bioanalysis Group, Departament d'Enginyeria Química, Universitat Rovira i Virgili , Avinguda Països Catalans 26, 43007 Tarragona, Spain.
Langmuir. 2015;31(1):535-41. doi: 10.1021/la504065r. Epub 2014 Dec 23.
Small carbon nano-onions (CNOs, 6-12 shells) were prepared in high yields and functionalized with carboxylic groups by chemical oxidation and reacted with βCD-NH2 to yield CNOs decorated with βCDs. A biocompatibile dextran polymer with graphted ferrocene groups was employed for the supramolecular self-assembly on the βCD-CNO surfaces. The βCDs act as hosts and the polymer ferrocene groups as guests (Fc-Dex) by the formation of inclusion complexes. After their assembly these nanostructures were soluble in aqueous solutions. The resulting product was characterized by transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and FT-IR and Raman spectroscopies. Moreover, the deposition of successive layers on the surface of the particles was monitored using DLS measurements and zeta potentials. Through-space interactions between the Fc moieties and the CNO cores and the influence of an additional dextran-βCD outer layer were measured electrochemically.
高产率制备了小尺寸碳纳米洋葱（CNOs，6 - 12个壳层），通过化学氧化使其羧基官能化，并与βCD - NH₂反应，得到用βCD修饰的CNOs。一种带有接枝二茂铁基团的生物相容性葡聚糖聚合物用于在βCD - CNO表面进行超分子自组装。通过形成包合物，βCD充当主体，聚合物二茂铁基团充当客体（Fc - Dex）。组装后，这些纳米结构可溶于水溶液。所得产物通过透射电子显微镜（TEM）、热重分析（TGA）以及傅里叶变换红外光谱（FT - IR）和拉曼光谱进行表征。此外，使用动态光散射（DLS）测量和zeta电位监测颗粒表面连续层的沉积。通过电化学方法测量了Fc部分与CNO核心之间的空间相互作用以及额外的葡聚糖 - βCD外层的影响。
