Dankner W M, Spector S A
Department of Pediatrics, University of California, San Diego 92103.
Am J Anat. 1989 Jun-Jul;185(2-3):310-3. doi: 10.1002/aja.1001850224.
Human cytomegalovirus (HCMV) is an important pathogen in neonates, transplant patients, and individuals with acquired immunodeficiency syndrome (AIDS). Reliable techniques for the detection of this virus in clinical specimens would aid in improving methods for diagnosis and increasing our understanding of viral pathogenesis. We evaluated the utility of immunogold-silver enhancement to determine the specificity of murine monoclonal antibodies directed against HCMV proteins and applied these antibodies to the detection of HCMV in histologic material. Nine antibodies were tested in a tissue-culture system to determine the location of staining. All were found to be active in frozen tissue, but only two of these antibodies were reactive in formalin-fixed tissue. We also evaluated a novel tissue fixative technique (AMeX; Fixation and dehydration with acetone) that has been used to maintain antigenicity of T-lymphocyte cell markers. All antibodies remained reactive against their respective HCMV proteins in tissue fixed by this technique. However, dehydration of tissue may limit the usefulness of AMeX fixation. Immunogold-silver enhancement is a useful and reliable histochemical technique for detection of HCMV antigens in pathologic specimens.
人巨细胞病毒（HCMV）是新生儿、移植患者及获得性免疫缺陷综合征（AIDS）患者的重要病原体。临床标本中检测该病毒的可靠技术将有助于改进诊断方法并增进我们对病毒发病机制的理解。我们评估了免疫金银染色增强法在确定针对HCMV蛋白的鼠单克隆抗体特异性方面的效用，并将这些抗体应用于组织学材料中HCMV的检测。在组织培养系统中检测了九种抗体以确定染色位置。发现所有抗体在冷冻组织中均有活性，但其中只有两种抗体在福尔马林固定组织中有反应性。我们还评估了一种新型组织固定技术（AMeX；用丙酮固定和脱水），该技术已用于维持T淋巴细胞细胞标志物的抗原性。在通过该技术固定的组织中，所有抗体对其各自的HCMV蛋白仍有反应性。然而，组织脱水可能会限制AMeX固定的实用性。免疫金银染色增强法是一种用于在病理标本中检测HCMV抗原的有用且可靠的组织化学技术。
