人巨细胞病毒的包涵体由病毒的DNA以及即刻早期抗原和早期抗原组成。
[Inclusion bodies of human cytomegalovirus are composed of the DNA and immediately early and early antigens of the virus].
作者信息
Yan Qing-guo, Huang Gao-sheng, Guo Ying, Wang Zhe, Feng Ji-liang, Yang Guo-rong
机构信息
Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Sep;17(3):262-5.
BACKGROUND
To study the composition and significance of the inclusion bodies of human cytomegalovirus (HCMV).
METHODS
Microdissection of inclusion bodies, PCR and Southern blot were adopted to detect DNA, and immunohistochemistry method and catalyzed signal amplification (CSA) were used to detect the different antigens of HCMV.
RESULTS
The inclusion bodies of HCMV were separated from the tissue section of human salivary gland. The fragments amplified by PCR from these dissected inclusion bodies were confirmed to be the DNA of HCMV. With the immunohistochemical method CSA, the immediately early and early antigens of HCMV were detected with monoclonal antibodies DDG9/CCH2, while matrix protein AAC10 was negative in the inclusion bodies.
CONCLUSION
The ingredient of inclusion bodies of HCMV included the DNA and the antigens expressed in specific stage of the virus.
背景
研究人巨细胞病毒（HCMV）包涵体的组成及意义。
方法
采用包涵体显微切割、PCR及Southern印迹法检测DNA，应用免疫组织化学方法及催化信号放大（CSA）法检测HCMV的不同抗原。
结果
从人唾液腺组织切片中分离出HCMV包涵体。经PCR从这些切割的包涵体中扩增出的片段被证实为人巨细胞病毒的DNA。采用免疫组织化学CSA法，用单克隆抗体DDG9/CCH2检测到HCMV的即刻早期和早期抗原，而包涵体中的基质蛋白AAC10呈阴性。
结论
HCMV包涵体的成分包括DNA及病毒特定阶段表达的抗原。
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