Panasiewicz G, Zamojska A, Bieniek M, Gizejewski Z, Szafranska B
Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego Str 1A, 10-719 Olsztyn-Kortowo, Poland.
Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, Poland.
Anim Reprod Sci. 2015 Jan;152:123-36. doi: 10.1016/j.anireprosci.2014.11.012. Epub 2014 Dec 2.
This study describes the diversity of vestigial male uteri of the European bison (Eb) examined for: (1) morphology, (2) glycoprotein localization, (3) total protein and glycoprotein profiles, (4) steroid concentrations, and (5) PMDS based on the mutation of AMH and AMHR2 genes. Uteri of adult bulls (5-12 years old) were compared to a uterus of a juvenile female (6 months old). Male uterine proteins were analyzed in parallel to secretory endometrial proteins of pseudo-pregnant pig (PsEND) and BSA used as profile-controls. Hematoxylin/eosin-staining revealed the diversity of male uterine morphology, including lumen size/shape, endometrial (END) gland density, luminal knob-like epithelial structures and multiple intrauterine cells proliferating within the lumen. PAS-staining revealed the presence of glycoproteins restricted to luminal epithelial cells and END glands. Heterologous total protein PAGE-profiles (20-66kDa) revealed two dominant fractions (66 and 45kDa), similar to PAS-profiles (67 and 47kDa) in male and female uterine tissues. In male uterine tissues, androstendione and progesterone, but not testosterone, estrone or estradiol concentrations were lower than in the female. Sequencing of AMH- and AMHR2-like amplicons allowed identification of these gene mutations in Eb. Our results provide novel data regarding PMDS, demonstrating the diversity of uterine morphology, glycoprotein mass/profile, steroid concentration and AMH/AMHR2 mutations in Eb bulls.
本研究描述了对欧洲野牛（Eb）残留雄性子宫的多样性进行的检测，检测内容包括：（1）形态学，（2）糖蛋白定位，（3）总蛋白和糖蛋白谱，（4）类固醇浓度，以及（5）基于抗缪勒管激素（AMH）和抗缪勒管激素受体2（AMHR2）基因突变的持久性苗勒管综合征（PMDS）。将成年公牛（5至12岁）的子宫与一头幼年雌性（6个月大）的子宫进行比较。同时分析雄性子宫蛋白以及用作谱对照的假孕猪分泌型子宫内膜蛋白（PsEND）和牛血清白蛋白（BSA）。苏木精/伊红染色揭示了雄性子宫形态的多样性，包括管腔大小/形状、子宫内膜（END）腺体密度、管腔结节状上皮结构以及管腔内增殖的多个子宫内细胞。过碘酸希夫（PAS）染色显示糖蛋白仅存在于管腔上皮细胞和END腺体中。异源总蛋白聚丙烯酰胺凝胶电泳谱（20至66 kDa）显示出两个主要组分（66和45 kDa），类似于雄性和雌性子宫组织中的PAS谱（67和47 kDa）。在雄性子宫组织中，雄烯二酮和孕酮的浓度低于雌性，但睾酮、雌酮或雌二醇的浓度并非如此。对类似AMH和AMHR2扩增子的测序使得能够在欧洲野牛中鉴定出这些基因突变。我们的结果提供了关于PMDS的新数据，证明了欧洲野牛公牛子宫形态、糖蛋白质量/谱、类固醇浓度和AMH/AMHR2突变的多样性。
