School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Food & Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Carbohydr Polym. 2015 Mar 6;117:771-777. doi: 10.1016/j.carbpol.2014.10.048. Epub 2014 Oct 30.
This study demonstrates a facile, green strategy for the preparation of gold nanoparticles (AuNPs) from chloroauric acid (HAuCl4) using carboxylic curdlan (Cc) as both reducing and stabilizing agent. The as-prepared AuNPs are characterized by UV-vis spectroscopy, high resolution transmission electron microscopy, X-ray diffraction, energy dispersive X-ray spectrometry and Fourier transform infrared spectroscopy. The results indicated that the particle size of the AuNPs changes with variations in the reaction time and concentrations of Cc and HAuCl4. The spherical AuNPs are well dispersed, exhibiting high stability even after six months storage. The carboxylic groups (COO(-)) in the Cc molecules tend to adsorb and stabilize the surface of the AuNPs. The interaction between BSA and the Cc-capped AuNPs was investigated using fluorescence and circular dichroism spectroscopies. The results indicated that the BSA molecules adsorb on the surface of the AuNPs, without significant change in its helical structure even after conjugation with the AuNPs.
本研究展示了一种简便、绿色的策略，使用羧甲基(curda)lan (Cc) 作为还原剂和稳定剂，从氯金酸 (HAuCl4) 制备金纳米粒子 (AuNPs)。所制备的 AuNPs 通过紫外-可见光谱、高分辨率透射电子显微镜、X 射线衍射、能量色散 X 射线光谱和傅里叶变换红外光谱进行表征。结果表明，AuNPs 的粒径随反应时间和 Cc 和 HAuCl4 浓度的变化而变化。球形 AuNPs 分散良好，即使在储存六个月后仍表现出高稳定性。Cc 分子中的羧基 (COO(-)) 倾向于吸附和稳定 AuNPs 的表面。使用荧光和圆二色光谱研究了 BSA 与 Cc 封端的 AuNPs 之间的相互作用。结果表明，BSA 分子吸附在 AuNPs 表面上，即使与 AuNPs 结合后，其螺旋结构也没有明显变化。
