Ristau E, Wittmann W, Starick E, Kluge K H
Arch Exp Veterinarmed. 1989 Jan;43(1):155-8.
8 calves were vaccinated 3 times with 1.5.10(7) BL-3 cells. 2 and 6 weeks, respectively, after the 3rd vaccination each 4 calves were subjected to a challenge infection with 2.5.10(5) blood lymphocytes of a BLV-infected cow. For control a challenge infection was performed on 3 non-vaccinated calves. The vaccination caused in all calves the appearance of complement-dependent cytotoxic antibodies against BL-3 cells but not of antibodies against BLV-gp51. After the challenge infection there occurred in the sera of all vaccinated calves and of all non-vaccinated control animals antibodies against gp51, and BLV could be demonstrated in the cultivated blood lymphocytes of all animals. Consequently, the application of BL-3 cells had no protective effect against the BLV infection.
8头小牛用1.5×10⁷个BL - 3细胞进行了3次接种。在第3次接种后分别于2周和6周，每组4头小牛用来自一头感染BLV的奶牛的2.5×10⁵个血液淋巴细胞进行攻击感染。作为对照，对3头未接种的小牛进行了攻击感染。接种在所有小牛中引起了针对BL - 3细胞的补体依赖性细胞毒性抗体的出现，但未引起针对BLV - gp51的抗体出现。攻击感染后，所有接种小牛和所有未接种对照动物的血清中都出现了针对gp51的抗体，并且在所有动物培养的血液淋巴细胞中都检测到了BLV。因此，应用BL - 3细胞对BLV感染没有保护作用。
