Burkhardt H, Rosenthal S, Wittmann W, Starick E, Scholz D, Rosenthal H A, Kluge K H
Arch Exp Veterinarmed. 1989 Nov;43(6):933-42.
Calves were immunised by means of a native Al(OH)3-absorbed gp51-preparation which had been obtained from a foetal lamb kidney cell line, following bovine leucosis virus (BLV) infection. 9 animals were immunised 3 times, using 300 micrograms gp51. 7 animals underwent test infection, using 2.5 x 10(3) or 2.5 x 10(4) BLV-infected lymphocytes. Serological and virological reactions of all animals, including 3 calves which had received only test infections, were followed up through 40 weeks by means of immunodiffusion test, enzyme-immuno-assay, gp51 antibody radio-immuno-assay, reverse transcriptase test, syncytial test, competitive p24 radio-immuno-assay, and by transmission of whole blood in animal experiments. The results obtained from virological testing showed that 1 animal had been protected by preceding immunisation. 4 in 7 immunised and test-infected animals exhibited transient BLV values, between the 7th and 16th weeks from infection. Typical leucosis infection had been induced to 2 animals. The above findings are discussed and are compared to similar results recorded by other working groups.
用从感染牛白血病病毒（BLV）的胎羊肾细胞系中获得的天然氢氧化铝吸附的gp51制剂对犊牛进行免疫。9只动物用300微克gp51免疫3次。7只动物用2.5×10³或2.5×10⁴个感染BLV的淋巴细胞进行试验感染。通过免疫扩散试验、酶免疫测定、gp51抗体放射免疫测定、逆转录酶试验、合胞体试验、竞争性p24放射免疫测定以及在动物实验中进行全血传播，对所有动物（包括仅接受试验感染的3头犊牛）的血清学和病毒学反应进行了40周的跟踪。病毒学检测结果表明，1只动物因先前的免疫而得到保护。7只免疫和试验感染的动物中有4只在感染后第7至16周出现短暂的BLV值。2只动物诱发了典型的白血病感染。对上述发现进行了讨论，并与其他工作组记录的类似结果进行了比较。
