Laychock S G
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0524.
Biochem J. 1989 May 15;260(1):291-4. doi: 10.1042/bj2600291.
Isolated islets of the rat labelled with myo-[3H]inositol showed decreased accumulation of total inositol phosphates (InsPs) and [3H]polyphosphoinositide hydrolysis in response to glucose after preincubation with prostaglandin E2 (PGE2). The response was concentration-dependent and specific for PGE2. PGE2 did not affect basal [3H]phosphoinositide hydrolysis or InsPs accumulation. Pertussis-toxin pretreatment antagonized the response to PGE2, whereas 8-bromo cyclic AMP was without effect. The PGE2-induced decrease in InsPs may contribute to the suppression of insulin secretion.
用肌醇-[3H]标记的大鼠分离胰岛在与前列腺素E2(PGE2)预孵育后,对葡萄糖的反应显示总肌醇磷酸酯(InsPs)积累减少和[3H]多磷酸肌醇水解减少。该反应呈浓度依赖性且对PGE2具有特异性。PGE2不影响基础[3H]磷酸肌醇水解或InsPs积累。百日咳毒素预处理可拮抗对PGE2的反应,而8-溴环磷酸腺苷则无作用。PGE2诱导的InsPs减少可能有助于抑制胰岛素分泌。