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非洲爪蟾卵母细胞生发泡中ATP+Mg依赖性和多阳离子刺激的蛋白磷酸酶的鉴定。

Identification of the ATP + Mg-dependent and polycation-stimulated protein phosphatases in the germinal vesicle of the Xenopus oocyte.

作者信息

Jessus C, Goris J, Staquet S, Cayla X, Ozon R, Merlevede W

机构信息

Laboratoire de Physiologie de la Reproduction, Université Pierre et Marie Curie, Paris, France.

出版信息

Biochem J. 1989 May 15;260(1):45-51. doi: 10.1042/bj2600045.

Abstract

Two protein phosphatase activities were characterized in the germinal vesicle of Xenopus laevis oocytes after manual dissection of the nucleus. One enzyme can be classified as an active form of the ATP + Mg-dependent (AMD) phosphatase, the other as a polycation-stimulated (PCS) phosphatase. The activity of the PCS phosphatase is localized exclusively in the soluble compartment of the nucleus (nucleoplasm). The catalytic subunit of the AMD phosphatase activity is associated either with the nuclear particulate fraction or with an inhibitory subunit in the nucleoplasm.

摘要

在对非洲爪蟾卵母细胞核进行手工解剖后,在其生发泡中鉴定出了两种蛋白质磷酸酶活性。一种酶可归类为ATP + Mg依赖性(AMD)磷酸酶的活性形式,另一种为聚阳离子刺激(PCS)磷酸酶。PCS磷酸酶的活性仅定位于细胞核的可溶性部分(核质)。AMD磷酸酶活性的催化亚基与核颗粒部分或核质中的抑制亚基相关联。

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本文引用的文献

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Nuclear protein kinases.核蛋白激酶
Mol Cell Biochem. 1984;59(1-2):81-99. doi: 10.1007/BF00231306.
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Release and activation of phosphorylase phosphatase upon rupture of organelles from rat liver.
Biochem Biophys Res Commun. 1982 Jan 29;104(2):443-50. doi: 10.1016/0006-291x(82)90657-x.
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Nonhistone chromosomal proteins and gene regulation.非组蛋白染色体蛋白与基因调控。
Science. 1974 Mar 1;183(4127):817-24. doi: 10.1126/science.183.4127.817.

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