Huang Mingchao, Wang Yuyu, Liu Xingyue, Li Weihai, Kang Zehui, Wang Kai, Li Xuankun, Yang Ding
Department of Entomology, China Agricultural University, Beijing 100193, China.
Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang, China.
Gene. 2015 Feb 15;557(1):52-60. doi: 10.1016/j.gene.2014.12.009. Epub 2014 Dec 9.
The Plecoptera (stoneflies) is a hemimetabolous order of insects, whose larvae are usually used as indicators for fresh water biomonitoring. Herein, we describe the complete mitochondrial (mt) genome of a stonefly species, namely Acroneuria hainana Wu belonging to the family Perlidae. This mt genome contains 13 PCGs, 22 tRNA-coding genes and 2 rRNA-coding genes that are conserved in most insect mt genomes, and it also has the identical gene order with the insect ancestral gene order. However, there are three special initiation codons of ND1, ND5 and COI in PCGs: TTG, GTG and CGA, coding for L, V and R, respectively. Additionally, the 899-bp control region, with 73.30% A+T content, has two long repeated sequences which are found at the 3'-end closing to the tRNA(Ile) gene. Both of them can be folded into a stem-loop structure, whose adjacent upstream and downstream sequences can be also folded into stem-loop structures. It is presumed that the four special structures in series could be associated with the D-loop replication. It might be able to adjust the replication speed of two replicate directions.
襀翅目（石蝇）是昆虫的一个渐变态目，其幼虫通常用作淡水生物监测的指标。在此，我们描述了一种石蝇物种海南钩襀（Acroneuria hainana Wu）的完整线粒体（mt）基因组，该物种属于网襀科。这个线粒体基因组包含13个蛋白质编码基因（PCGs）、22个编码tRNA的基因和2个编码rRNA的基因，这些基因在大多数昆虫线粒体基因组中是保守的，并且其基因排列顺序与昆虫祖先的基因排列顺序相同。然而，蛋白质编码基因中ND1、ND5和COI有三个特殊的起始密码子：TTG、GTG和CGA，分别编码L、V和R。此外，899 bp的控制区，A + T含量为73.30%，有两个长重复序列，位于靠近tRNA（Ile）基因的3'端。它们都可以折叠成茎环结构，其相邻的上游和下游序列也可以折叠成茎环结构。推测这四个串联的特殊结构可能与D环复制有关。它可能能够调节两个复制方向的复制速度。
