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石蝇(襀翅目:石蝇科)的线粒体全基因组分析

The complete mitochondrial genome analysis of the stonefly, sp. (Plecoptera: Perlidae).

作者信息

Wang Ying, Cao Jinjun, Chen Mengdan, Li Weihai

机构信息

Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang, P. R. China.

出版信息

Mitochondrial DNA B Resour. 2019 Nov 8;4(2):3902-3903. doi: 10.1080/23802359.2019.1687032.

Abstract

is a small genus in Acroneuriinae, which was established by Chu but was treated as a synonym for a long time until Uchida and Harper recognized as a valid genus. This genus includes 15 species in the world now, including six species from China. Here, we sequenced and annotated the mitochondrial genome of sp. under the next-generation sequence technology, the first representative of Genus . The complete mitochondrial genome of sp. is 15,796 bp in length with an A + T content of 68.3% showed a positive AT-skew (0.037) and a negative GC-skew (-0.262). The gene order and organization of the mitochondrial genome is consistent with other stoneflies. The control region had the highest A + T content. Eleven PCGs started with the typical codon (ATN), the remaining PCGs started with TTG (ND1and ND5). All PCGs terminated with TAA/TAG, except COII and ND5 used single T. The phylogenetic tree by maximum likelihood (ML) and Bayesian (BI) methods supported that sp. was the sister group to .

摘要

是石蝇科中的一个小属,由Chu建立,但在很长一段时间内被视为同义词,直到Uchida和Harper将其确认为一个有效属。该属目前世界上有15个物种,其中6种来自中国。在此,我们在新一代测序技术下对该属的 种的线粒体基因组进行了测序和注释,它是该属的首个代表物种。该种的完整线粒体基因组长度为15,796 bp,A+T含量为68.3%,呈现正的AT偏斜(0.037)和负的GC偏斜(-0.262)。线粒体基因组的基因顺序和组织与其他石蝇一致。控制区的A+T含量最高。11个蛋白质编码基因(PCGs)以典型密码子(ATN)起始,其余PCGs以TTG起始(ND1和ND5)。除COII和ND5使用单个T外,所有PCGs均以TAA/TAG终止。通过最大似然法(ML)和贝叶斯法(BI)构建的系统发育树支持该种是 的姐妹群。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b02a/7707452/61f25109494c/TMDN_A_1687032_F0001_B.jpg

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