Gong Guanghui, Chen Puxiang, Li Long, Tan Hong, Zhou Jun, Zhou Yihong, Yang Xiaojing, Wu Xiaoying
Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, PR China.
Department of Gynecology and Obstetrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, PR China.
Pathol Res Pract. 2015 Feb;211(2):175-82. doi: 10.1016/j.prp.2014.11.008. Epub 2014 Nov 26.
To elucidate the different roles of lamin A and lamin C in the metastasis of epithelial ovarian cancer (EOC) by examining their expression in EOC tissues and cell lines and their correlations with clinicopathological features.
The expression of lamin A and lamin C was assessed in ovarian tissues from 61 EOC patients and 13 normal, 14 benign controls, respectively, using immunohistochemistry. Two pairs of EOC cell lines: HO-8910, HO-8910PM, SKOV-3 and SKOV-3ip were also examined to see the differential expression patterns of lamin A and lamin C.
Lamin A expression was significantly lower in EOC tissues than that in normal and benign ovarian tissues (p<0.05), while lamin C expression was not. Lamin A expression level was closely associated with pathological grades, clinical stages, peritoneal metastasis and lymph node metastasis (all p<0.05). The progression-free survival rate of the EOC patients with low lamin A expression level was remarkably lower than that of the EOC patients with high expression level (p<0.05). Lamin A detected by PCR, Western Blot and immunocytochemistry also showed a significantly lower expression level in HO-8910PM than that in HO-8910 (p<0.05), but not for lamin C. There was no difference between SKOV-3 and SKOV-3ip cells.
The results suggested that loss of lamin A but not lamin C expression in EOC was related to metastasis and poor prognosis. Lamin A may play a critical role in the metastasis of EOC.
通过检测层粘连蛋白A（lamin A）和层粘连蛋白C（lamin C）在上皮性卵巢癌（EOC）组织和细胞系中的表达及其与临床病理特征的相关性，阐明它们在EOC转移中的不同作用。
采用免疫组织化学法分别检测61例EOC患者、13例正常对照和14例良性对照的卵巢组织中层粘连蛋白A和层粘连蛋白C的表达。还检测了两对EOC细胞系：HO-8910、HO-8910PM、SKOV-3和SKOV-3ip，以观察层粘连蛋白A和层粘连蛋白C的差异表达模式。
EOC组织中层粘连蛋白A的表达明显低于正常和良性卵巢组织（p<0.05），而层粘连蛋白C的表达则无此差异。层粘连蛋白A的表达水平与病理分级、临床分期、腹膜转移和淋巴结转移密切相关（均p<0.05）。层粘连蛋白A表达水平低的EOC患者的无进展生存率明显低于表达水平高的EOC患者（p<0.05）。通过PCR、Western Blot和免疫细胞化学检测发现，HO-8910PM中层粘连蛋白A的表达水平也明显低于HO-8910（p<0.05），但层粘连蛋白C无此差异。SKOV-3和SKOV-3ip细胞之间无差异。
结果表明，EOC中层粘连蛋白A而非层粘连蛋白C的表达缺失与转移和预后不良有关。层粘连蛋白A可能在EOC转移中起关键作用。
