利用产丁酸梭菌从微藻生物柴油残渣中生产生物丁醇。
Biological butanol production from microalgae-based biodiesel residues by Clostridium acetobutylicum.
机构信息
Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan.
Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan; Sustainable Environment Research Laboratory (SERL), National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan; Research Center for Energy Technology and Strategy (RCETS), National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan.
出版信息
Bioresour Technol. 2015 May;184:379-385. doi: 10.1016/j.biortech.2014.11.017. Epub 2014 Nov 18.
This study conducted batch experiments to evaluate the potential of butanol production from microalgae biodiesel residues by Clostridium acetobutylicum. The results indicated that with 90 g/L of glucose as the sole substrate the highest butanol yield of 0.2 g/g-glucose was found, but the addition of butyrate significantly enhanced the butanol yield. The highest butanol yield of 0.4 g/g-glucose was found with 60 g/L of glucose and 18 g/L of butyrate. Using microalgae biodiesel residues as substrate, C. acetobutylicum produced 3.86 g/L of butanol and achieved butanol yield of 0.13 g/g-carbohydrate via ABE fermentation, but the results indicated that approximately one third of carbohydrate was not utilized by C. acetobutylicum. Biological butanol production from microalgae biodiesel residues can be possible, but further research on fermentation strategies are required to improve production yield.
本研究通过丙酮丁醇梭菌进行批式实验，评估了利用微藻生物柴油残渣生产丁醇的潜力。结果表明，以 90g/L 的葡萄糖作为唯一底物时，最高丁醇得率为 0.2g/g-葡萄糖，但添加丁酸可显著提高丁醇得率。当葡萄糖浓度为 60g/L，丁酸浓度为 18g/L 时，丁醇得率最高，达到 0.4g/g-葡萄糖。以微藻生物柴油残渣为底物，丙酮丁醇梭菌通过 ABE 发酵生产了 3.86g/L 的丁醇，丁醇得率为 0.13g/g-碳水化合物，但结果表明，大约有三分之一的碳水化合物未被丙酮丁醇梭菌利用。从微藻生物柴油残渣中进行生物丁醇生产是可行的，但需要进一步研究发酵策略，以提高生产产量。
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