College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, Maryland, USA.
Microb Biotechnol. 2023 Feb;16(2):448-462. doi: 10.1111/1751-7915.14100. Epub 2022 Aug 1.
Certain microalgal species can grow under high CO concentrations providing potential for mitigating CO pollution in flue gas produced by power plants. Microalga Scenedesmus obliquus strain HTB1 was isolated from the Chesapeake Bay and grow rapidly in a high level of CO . However, little is known about the molecular responses of HTB1 to high CO levels. Here, we investigated how HTB1 responds to 10% CO using the time-resolved transcriptome analysis. Gene expression profiles indicated that HTB1 responds quickly (in 2 h) and becomes adaptive within 12 h when exposed to 10% CO . Interestingly, certain genes of light-harvesting, chlorophyll synthesis and carbon fixation (i.e. rbcS) were up-regulated at 10% CO , and these functional responses are consistent with the increased photosynthesis efficiency and algal biomass under 10% CO . Nitrate assimilation was strongly enhanced, with amino acid biosynthesis and aminoacyl tRNA biosynthesis genes being markedly up-regulated, indicating that HTB1 actively takes up nitrogen and accelerates protein synthesis at 10% CO . Carbon metabolism including fatty acid biosynthesis and TCA cycle was enhanced at 10% CO , supporting the earlier observation of increased lipid content of Scenedesmus sp. under high CO levels. Interestingly, key genes like RuBisCO (rbcL) and carbonic anhydrase in carboxysomes did not respond actively to 10% CO , implying that exposure to 10% CO has little impact on the carbon concentrating mechanisms and CO fixation of the Calvin cycle. It appears that HTB1 can grow rapidly at 10% CO without significant metabolic changes in carbon fixation and ATP synthesis.
某些微藻物种可以在高浓度 CO 下生长，为减轻发电厂烟气中 CO 污染提供了潜力。从切萨皮克湾分离出的斜生栅藻 HTB1 可以在高浓度 CO 下快速生长。然而，关于 HTB1 对高 CO 水平的分子响应知之甚少。在这里，我们使用时间分辨转录组分析研究了 HTB1 对 10%CO 的反应。基因表达谱表明，当暴露于 10%CO 时，HTB1 会迅速（在 2 小时内）做出反应，并在 12 小时内变得适应。有趣的是，某些光捕获、叶绿素合成和碳固定（即 rbcS）的基因在 10%CO 下上调，这些功能反应与 10%CO 下光合作用效率和藻类生物量的增加一致。硝酸盐同化作用得到了强烈增强，氨基酸合成和氨酰 tRNA 合成基因明显上调，表明 HTB1 在 10%CO 下积极吸收氮并加速蛋白质合成。碳代谢包括脂肪酸合成和 TCA 循环在 10%CO 下增强，支持 Scenedesmus sp.在高 CO 水平下脂质含量增加的早期观察结果。有趣的是，像 RuBisCO（rbcL）和羧化体中的碳酸酐酶这样的关键基因对 10%CO 没有积极响应，这意味着暴露于 10%CO 对卡尔文循环的碳浓缩机制和 CO 固定影响不大。似乎 HTB1 可以在 10%CO 下快速生长，而碳固定和 ATP 合成没有明显的代谢变化。
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