Porras A, Fernández M, Benito M
Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Ciudad Universitaria, Madrid, Spain.
Biochem Biophys Res Commun. 1989 Aug 30;163(1):541-7. doi: 10.1016/0006-291x(89)92171-2.
The adrenergic and T3 modulation of UCP expression in non-proliferative foetal brown adipocyte primary cultures were studied. The UCP in the cultured cells was determined by immunological detection of the protein and by quantification of the mitochondrial GDP-binding. Our results showed a relative increase of 65-75% in UCP levels and 60-80% in the mitochondrial GDP-binding capacity under beta-adrenergic stimulatory conditions, while neither alpha 1-adrenergic agonists nor T3 showed an effect.
研究了非增殖性胎儿棕色脂肪细胞原代培养物中去甲肾上腺素能和T3对解偶联蛋白（UCP）表达的调节作用。通过蛋白质的免疫检测和线粒体GDP结合定量来测定培养细胞中的UCP。我们的结果表明，在β-肾上腺素能刺激条件下，UCP水平相对增加65 - 75%，线粒体GDP结合能力相对增加60 - 80%，而α1-肾上腺素能激动剂和T3均未显示出作用。
