Guerra C, Roncero C, Porras A, Fernández M, Benito M
Departamento de Bioquimica y Biologia Molecular II, Universidad Completense Facultad de Farmacia, Madrid, Spain.
J Biol Chem. 1996 Jan 26;271(4):2076-81. doi: 10.1074/jbc.271.4.2076.
Confluent fetal rat brown adipocytes in primary culture showed an almost undetectable level of uncoupling protein (UCP) mRNA and a low mitochondrial content of functional UCP. Treatment of confluent cells with 10 nM triiodothyronine in a serum-free medium, in the absence of noradrenergic stimulation, increased the amount of UCP mRNA in a time-dependent manner. This effect was due to an increased UCP gene transcription rate and UCP mRNA stabilization, resulting in a higher content of immunoreactive mitochondrial UCP and functional UCP (detected by its ability to bind GDP). Thus, triiodothyronine might play a significant physiological role in the UCP expression throughout fetal development, when brown adipose tissue starts to differentiate and UCP is primarily expressed.
原代培养的融合胎鼠棕色脂肪细胞显示出几乎检测不到的解偶联蛋白（UCP）mRNA水平以及功能性UCP的低线粒体含量。在无血清培养基中，在无去甲肾上腺素能刺激的情况下，用10 nM三碘甲状腺原氨酸处理融合细胞，UCP mRNA的量随时间依赖性增加。这种效应是由于UCP基因转录速率增加和UCP mRNA稳定性增加，导致免疫反应性线粒体UCP和功能性UCP（通过其结合GDP的能力检测）含量更高。因此，在棕色脂肪组织开始分化且UCP主要表达的整个胎儿发育过程中，三碘甲状腺原氨酸可能在UCP表达中发挥重要的生理作用。
