胆囊收缩素可提高小细胞肺癌细胞中的胞质钙水平。

Cholecystokinin elevates cytosolic calcium in small cell lung cancer cells.

作者信息

Staley J, Fiskum G, Moody T W

机构信息

Department of Biochemistry and Molecular Biology, George Washington University School of Medicine and Health Sciences, Washington, D.C. 20037.

出版信息

Biochem Biophys Res Commun. 1989 Aug 30;163(1):605-10. doi: 10.1016/0006-291x(89)92180-3.

DOI:10.1016/0006-291x(89)92180-3

PMID:2550000

Abstract

The ability of cholecystokinin (CCK) to elevate intracellular Ca2+ levels in small cell lung cancer cells was investigated using the fluorescent Ca2+ indicator Fura 2. CCK-8 elevated the cytosolic Ca2+ levels in cell line NCI-H345 in a dose dependent manner. Nanomolar concentration of CCK-8 elevated cytosolic Ca2+ levels in the absence or presence of extracellular Ca2+. Potent CCK agonists such as gastrin-1 and nonsulfated CCK-8 but not inactive compounds such as CCK-27-32-NH2 elevated cytosolic Ca2+ levels. These data suggest that CCK receptors may regulate the release of Ca2+ from intracellular organelles in small cell lung cancer cells.

摘要

使用荧光钙指示剂Fura 2研究了胆囊收缩素（CCK）提高小细胞肺癌细胞内钙离子水平的能力。CCK-8以剂量依赖的方式提高了细胞系NCI-H345中的胞质钙离子水平。在有无细胞外钙的情况下，纳摩尔浓度的CCK-8均可提高胞质钙离子水平。强效CCK激动剂如胃泌素-1和非硫酸化CCK-8可提高胞质钙离子水平，而无活性化合物如CCK-27-32-NH2则不能。这些数据表明，CCK受体可能调节小细胞肺癌细胞内细胞器中钙离子的释放。