神经降压素可提高小细胞肺癌细胞中的胞质钙水平。

Neurotensin elevates cytosolic calcium in small cell lung cancer cells.

作者信息

Staley J, Fiskum G, Davis T P, Moody T W

机构信息

Department of Biochemistry, George Washington University School of Medicine and Health Sciences, Washington, DC 20037.

出版信息

Peptides. 1989 Nov-Dec;10(6):1217-21. doi: 10.1016/0196-9781(89)90015-6.

DOI:10.1016/0196-9781(89)90015-6

PMID:2560176

Abstract

The ability of neurotensin (NT) to elevate cytosolic Ca2+ in small cell lung cancer (SCLC) cells was investigated using the fluorescent Ca2+ indicator Fura 2-AM. Using SCLC cell line NCI-H345, NT elevated cytosolic Ca2+ levels in a concentration-dependent manner. Using a 10 nM dose, NT and C-terminal fragments such as NT(8-13) but not N-terminal fragments such as NT(1-8) elevated the cytosolic Ca2+ levels. Because EGTA (5 mM) did not affect the NT response, NT may cause release of Ca2+ from intracellular stores. These data indicate that SCLC NT receptors may use Ca2+ as a second messenger.

摘要

使用荧光钙指示剂Fura 2-AM研究了神经降压素（NT）升高小细胞肺癌（SCLC）细胞胞质Ca2+的能力。使用SCLC细胞系NCI-H345，NT以浓度依赖性方式升高胞质Ca2+水平。使用10 nM剂量时，NT和C端片段如NT(8-13)可升高胞质Ca2+水平，但N端片段如NT(1-8)则不能。由于乙二醇双四乙酸（EGTA，5 mM）不影响NT反应，NT可能从细胞内储存库释放Ca2+。这些数据表明，SCLC的NT受体可能将Ca2+用作第二信使。