Laboratorio de Investigación Molecular en Nutrición (LIMON), Universidad del Centro de México UCEM, San Luis Potosí, México.
Laboratorio de Investigación Molecular en Nutrición (LIMON), Universidad del Centro de México UCEM, San Luis Potosí, México.
Arch Med Res. 2015 Jan;46(1):78-83. doi: 10.1016/j.arcmed.2014.12.001. Epub 2014 Dec 10.
The SNP rs662 in the paraoxonase 1 gene (PON1 Q192R) has been associated with obesity, dyslipidemia and cardiovascular risk. In this study, DNA samples of 117 children aged 6 to 12 years from San Luis Potosí, México were genotyped for Q192R polymorphism of the PON1 gene.
Genotypic frequencies were determined by allelic discrimination assay by real-time PCR using TaqMan fluorogenic probes. Anthropometry, lipid profile, glucose and insulin were analyzed by genotype.
The distribution of allele frequency in the population was Q = 65 and R = 35 following the Hardy Weinberg equilibrium (χ(2) = 3.15, p = 0.076). The Homeostasis Model Assessment for Insulin Resistance (HOMA-IR) index showed statistically significant differences among QQ/QR/RR genotypes (p = 0.032). The odds ratio for the carriers of the RR genotype was associated with HOMA-IR corresponding to the 95(th) percentile or higher for Mexican children based on sex and age (OR = 4.68; 95% confidence intervals, 1.23-17.8; p = 0.016). When the absolute mean of HOMAR-IR was set as the cutoff, an increased odds was observed (OR = 6.52; 95% confidence intervals, 1.68-25.3; p = 0.008).
According to our results, PON1 Q192R polymorphism is a risk marker for insulin resistance, a pathological factor involved in the development of metabolic syndrome.
载脂蛋白 1 基因（PON1 Q192R）中的 SNP rs662 与肥胖、血脂异常和心血管风险有关。在这项研究中，对来自墨西哥圣路易斯波托西的 117 名 6 至 12 岁的儿童的 DNA 样本进行了 PON1 基因 Q192R 多态性的基因分型。
通过实时 PCR 用 TaqMan 荧光探针进行等位基因鉴别分析确定基因型频率。根据基因型分析了人体测量学、血脂谱、葡萄糖和胰岛素。
人群中等位基因频率的分布为 Q = 65，R = 35，符合 Hardy Weinberg 平衡（χ(2) = 3.15，p = 0.076）。胰岛素抵抗的稳态模型评估（HOMA-IR）指数在 QQ/QR/RR 基因型之间显示出统计学上的显著差异（p = 0.032）。RR 基因型携带者的优势比与 HOMA-IR 相关，对应于基于性别和年龄的墨西哥儿童的第 95 百分位数或更高（OR = 4.68；95%置信区间，1.23-17.8；p = 0.016）。当将 HOMAR-IR 的绝对平均值设定为截止值时，观察到增加的优势（OR = 6.52；95%置信区间，1.68-25.3；p = 0.008）。
根据我们的结果，PON1 Q192R 多态性是胰岛素抵抗的风险标志物，胰岛素抵抗是代谢综合征发展过程中涉及的病理因素。
