Xiao L, Zhong Y P, Wang B, Wu T L
Legume Biotechnology Laboratory of School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China.
Legume Biotechnology Laboratory of School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, China
Genet Mol Res. 2014 Nov 7;13(4):9152-60. doi: 10.4238/2014.November.7.2.
Soybean aphid (SA: Aphis glycines Matsumura) is one of the most serious pests of soybean [Glycine max (L.) Merr.] worldwide. A single dominant gene was found to control SA resistance in soybean line P746, which exhibits antibiosis resistance. This study aimed to define the location of the SA resistance gene in P746. A F2:3 mapping population, including 312 individuals, was created based on the cross of P746 and 'Dongnong 47'. Combined with bulked segregant analysis, all of the 1015 simple sequence repeats (SSR) from the soybean consensus map were used to locate the tentative genomic region of the SA resistance gene in P746. The effort resulted in the mapping of R_P746, the SA resistance gene in P746, and was flanked on either side by Satt334 and Satt335 on chromosome 13. By chromosome walking with SSRs from BARCSOYSSR_1.0, R_P746 was mapped between BARCSOYSSR_13_1278 and BARCSOYSSR_13_1363, with distances of 4.2 and 2.6 cM, respectively. The results indicate that R_P746 might be different to the SA resistance genes previously reported. The markers that are closely linked to R_P746 are expected to be useful for marker assisted selection in future soybean aphid resistance breeding programs.
大豆蚜（SA：豆蚜，Aphis glycines Matsumura）是全球大豆[Glycine max (L.) Merr.]最严重的害虫之一。在具有抗生性的大豆品系P746中发现一个显性基因控制对大豆蚜的抗性。本研究旨在确定P746中大豆蚜抗性基因的位置。基于P746与‘东农47’的杂交构建了一个包含312个单株的F2:3作图群体。结合集群分离分析法，利用大豆整合图谱中的1015个简单序列重复（SSR）标记来定位P746中大豆蚜抗性基因的初步基因组区域。结果将P746中的大豆蚜抗性基因R_P746定位在13号染色体上，两侧分别为Satt334和Satt335。通过利用BARCSOYSSR_1.0中的SSR标记进行染色体步移，R_P746被定位在BARCSOYSSR_13_1278和BARCSOYSSR_13_1363之间，距离分别为4.2和2.6 cM。结果表明R_P746可能与先前报道的大豆蚜抗性基因不同。与R_P746紧密连锁的标记有望在未来的大豆抗蚜育种计划中用于标记辅助选择。
