在粳稻地方品种“薄稻”中鉴定赋予稻瘟病抗性的数量性状基因座。

Identification of quantitative trait loci conferring blast resistance in Bodao, a japonica rice landrace.

作者信息

Huan J, Bao Y M, Wu Y Y, Zeng G Y, He W W, Dang L L, Wang J F, Zhang H S

机构信息

State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing, China.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing, China

出版信息

Genet Mol Res. 2014 Nov 27;13(4):9756-65. doi: 10.4238/2014.November.27.3.

DOI:10.4238/2014.November.27.3

PMID:25501185

Abstract

Bodao, a japonica landrace from the Taihu Lake region of China, is highly resistant to most Chinese isolates of Magnaporthe oryzea, a form of rice blast. To effectively dissect the influence of genetics on this blast resistance, a population of 155 recombinant inbred lines (F2:8) derived from a cross of Bodao x Suyunuo was inoculated with 12 blast isolates. Using a quantitative trait locus (QTL) mapping approach, 13 QTL on chromosomes 1, 2, 9, 11, and 12 were detected from Bodao. Five QTL, including qtl11-1-1, qtl11-3-7, qtl11-4-9, qtl12-1-1, and qtl12-2-3, have not been previously reported. The qtl11-3-7 and qtl11-4-9 may be the two main effective QTL and resistant to 7 and 9 isolates, respectively. The results of the present study will be valuable for the fine mapping and cloning of these two new resistance genes.

摘要

“钵稻”是一种来自中国太湖地区的粳稻地方品种，对大多数中国稻瘟病菌株（一种稻瘟病形式）具有高度抗性。为了有效剖析遗传学对这种稻瘟病抗性的影响，用12个稻瘟病菌株接种了由“钵稻”与“苏御糯”杂交产生的155个重组自交系（F2:8）群体。采用数量性状位点（QTL）定位方法，从“钵稻”中检测到位于第1、2、9、11和12号染色体上的13个QTL。其中5个QTL，包括qtl11 - 1 - 1、qtl11 - 3 - 7、qtl11 - 4 - 9、qtl12 - 1 - 1和qtl12 - 2 - 3，此前未见报道。qtl11 - 3 - 7和qtl11 - 4 - 9可能是两个主要的有效QTL，分别对7个和9个菌株具有抗性。本研究结果对于这两个新抗性基因的精细定位和克隆具有重要价值。