Rahim H A, Bhuiyan M A R, Lim L S, Sabu K K, Saad A, Azhar M, Wickneswari R
Bioscience and Agrotechnology Division, Malaysian Nuclear Agency, Kajang, Selangor, Malaysia.
Genet Mol Res. 2012 Sep 13;11(3):3277-89. doi: 10.4238/2012.September.12.11.
Advanced backcross families derived from Oryza sativa cv MR219/O. rufipogon IRGC105491 were utilized for identification of quantitative trait loci (QTL) for blast resistance using simple sequence repeat markers. Two hundred and sixty-one BC(2)F(3) families were used to construct a linkage map, using 87 markers, which covered 2375.2 cM of 12 rice chromosomes, with a mean density of 27.3 cM. The families were evaluated in a greenhouse for resistance to blast disease caused by pathotypes P7.2 and P5.0 of Magnaporthe oryzae. Five QTLs (qBL5.1, qBL5.2, qBL6.1, qBL8.1, and qBL10.1) for pathotype P5.0 and four QTLs (qBL5.3, qBL5.4, qBL7.1, and qBL8.2) for pathotype P7.2 were identified using the BC(2)F(3) families. Another linkage map was also constructed based on 31 BC(2)F(5) families, using 63 SSR markers, which covered 474.9 cM of 9 rice chromosomes, with a mean density of 8.01 cM. Five suggestive QTLs (qBL11.2, qBL11.3, qBL12.1, qBL12.2, qBL12.3) and one putative QTL (qBL2.1) were identified for pathotype P7.2. Also, seven suggestive QTLs (qBL1.1, qBL2.2, qBL4.1, qBL4.2, qBL5.3, qBL8.3, and qBL11.1) were detected for pathotype P5.0. We conclude that there is a non-race-specific resistance spectrum of O. rufipogon against M. oryzae pathotypes.
利用源自水稻品种MR219/野生稻IRGC105491的高代回交群体，通过简单序列重复标记鉴定抗稻瘟病的数量性状位点（QTL）。使用261个BC(2)F(3)家系构建连锁图谱，共使用87个标记，覆盖12条水稻染色体的2375.2 cM，平均间距为27.3 cM。在温室中对这些家系进行稻瘟病菌P7.2和P5.0致病型引起的稻瘟病抗性评估。利用BC(2)F(3)家系鉴定出针对致病型P5.0的5个QTL（qBL5.1、qBL5.2、qBL6.1、qBL8.1和qBL10.1）以及针对致病型P7.2的4个QTL（qBL5.3、qBL5.4、qBL7.1和qBL8.2）。还基于31个BC(2)F(5)家系构建了另一个连锁图谱，使用63个SSR标记，覆盖9条水稻染色体的474.9 cM，平均间距为8.01 cM。鉴定出针对致病型P7.2的5个推测性QTL（qBL11.2、qBL11.3、qBL12.1、qBL12.2、qBL12.3）和1个假定QTL（qBL2.1）。此外，针对致病型P5.0检测到7个推测性QTL（qBL1.1、qBL2.2、qBL4.1、qBL4.2、qBL5.3、qBL8.3和qBL11.1）。我们得出结论，野生稻对稻瘟病菌致病型存在非小种特异性的抗性谱。
