Kuang M, Yang W H, Wang F, Xu H X, Wang Y Q, Zhou D Y, Fang D, Ma L, Feng X A
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, China
Genet Mol Res. 2014 Nov 27;13(4):9777-86. doi: 10.4238/2014.November.27.5.
We screened and assessed published cotton simple sequence repeat (SSR) primers to establish a set of core SSR markers suitable for cotton major cultivars in China and analyzed genetic diversity based on the core marker set. Using a stepwise screening strategy, 12 leading cultivars for preliminary screening and 96 cultivars for rescreening were evaluated. A total of 184 polymorphic SSR markers were initially screened from 3299 candidates, and a core set of 52 SSR markers with wide genome coverage (2 markers per chromosome) was obtained. Among 96 major cultivars, 273 amplification genotypes were generated using the core marker set. Polymorphism information content values ranged from 0.28-0.83, with an average value of 0.56. The core SSR marker set detected on denaturing polyacrylamide gel electrophoresis indicated that the band genotype was either a single or double band on conventional cultivars, while most were double bands (65.4%). Among 56 hybrids, the average heterozygosis rate was 35.8%, ranging from 7.1-55.4%. Eighteen of 96 cultivars had distinct band genotypes. The genetic diversity analyzed using the of NTSYS-pc V2.10 software indicated that the Yangtze River valley cotton region had the highest polymorphic level, followed by Xinjiang and then the Yellow River valley. The genetic basis of conventional cultivars was narrower than that of hybrids. The core marker set can be used for fingerprint construction, variety identification, and purity tests of major cotton cultivars in China.
我们筛选并评估了已发表的棉花简单序列重复（SSR）引物，以建立一套适用于中国棉花主要品种的核心SSR标记，并基于该核心标记集分析遗传多样性。采用逐步筛选策略，对12个主导品种进行初步筛选，对96个品种进行重新筛选。从3299个候选引物中初步筛选出184个多态性SSR标记，获得了一套基因组覆盖范围广（每条染色体2个标记）的52个SSR核心标记。在96个主要品种中，使用核心标记集产生了273种扩增基因型。多态性信息含量值在0.28 - 0.83之间，平均值为0.56。在变性聚丙烯酰胺凝胶电泳上检测到的核心SSR标记集表明，常规品种的条带基因型为单带或双带，而大多数为双带（65.4%）。在56个杂交种中，平均杂合率为35.8%，范围为7.1% - 55.4%。96个品种中有18个具有独特的条带基因型。使用NTSYS-pc V2.10软件分析的遗传多样性表明，长江流域棉区的多态性水平最高，其次是新疆，然后是黄河流域。常规品种的遗传基础比杂交种窄。该核心标记集可用于中国主要棉花品种的指纹图谱构建、品种鉴定和纯度检测。
