Maranho R C, Augusto R, Mangolin C A, Machado M F P S
Programa de Pós-Graduação em Agronomia, Universidade Estadual de Maringá, Maringá, PR, Brasil.
Programa de Pós-Graduação em Genética e Melhoramento, Universidade Estadual de Maringá, Maringá, PR, Brasil.
Genet Mol Res. 2014 Dec 4;13(4):10130-41. doi: 10.4238/2014.December.4.7.
In this study, we measured the genetic diversity within and among a set of 9 commercial sugarcane varieties used for alcohol and sugar production using 17 microsatellite DNA markers. The UGSM148 and UGSM59 primers were monomorphic for all 74 sugarcane samples. The estimated proportion of simple sequence repeated (SSR) polymorphic loci was 88.23%; 17 alleles were detected. The mean gene diversity of all SSR loci was 0.7279. The highest observed heterozygosity (HO) value was found in the RB72454 variety, whereas the lowest HO value was recorded in the SP813250 variety. The SP813250, RB845210, and RB835054 sugarcane varieties were the most genetically uniform varieties. An extremely high level of population differentiation was detected in the varieties exhibiting similar agronomic characteristics. Analysis of the genetic structure of the 9 sugarcane varieties using SSR markers was especially important to identify SSR loci with high levels of heterozygosity and to identify varieties showing the highest levels of heterozygosity. The monomorphic primers may be used to evaluate the genetic stability of sugarcane during cycles of vegetative multiplication, i.e., propagation via rhizomes.
在本研究中，我们使用17个微卫星DNA标记，测定了用于酒精和糖生产的9个商业甘蔗品种内部和之间的遗传多样性。UGSM148和UGSM59引物对所有74个甘蔗样品均为单态性。简单序列重复（SSR）多态性位点的估计比例为88.23%；共检测到17个等位基因。所有SSR位点的平均基因多样性为0.7279。在RB72454品种中观察到最高的杂合度（HO）值，而在SP813250品种中记录到最低的HO值。SP813250、RB845210和RB835054甘蔗品种是遗传上最均匀的品种。在表现出相似农艺特征的品种中检测到极高水平的群体分化。利用SSR标记分析9个甘蔗品种的遗传结构，对于鉴定具有高杂合度水平的SSR位点以及鉴定显示出最高杂合度水平的品种尤为重要。单态性引物可用于评估甘蔗在无性繁殖周期（即通过根茎繁殖）中的遗传稳定性。
