Nawaz S, Khan F A, Tabasum S, Iqbal M Z, Saeed A
CABB, University of Agriculture, Faisalabad, Pakistan.
Genet Mol Res. 2010 Jun 1;9(2):1011-22. doi: 10.4238/vol9-2gmr795.
Forty genotypes (clones) of sugarcane, including elite lines, commercial cultivars of Saccharum officinarum and clones of S. barberi were fingerprinted with 50 SSR markers using a PCR-based marker assay. Nei's genetic distances for SSR data were determined and relationships between accessions were portrayed graphically in the form of a dendrogram. Genetic distance values ranging from 0.60 to 1.11 were observed among the 40 sugarcane accessions. The shortest genetic distance of 0.60 was seen between genotypes US-804 and US-130. These two genotypes differed from each other only in 10 bands, with 20 primers. The most dissimilar of the accessions were CP-77-400 and US-133, with a genetic distance of 1.11. SSR fingerprints can help sugarcane breeders to clarify the genetic pedigree of commercial sugarcane varieties and evaluate the efficiency of breeding methods.
利用基于PCR的标记分析法，用50个SSR标记对40个甘蔗基因型（克隆）进行了指纹图谱分析，这些基因型包括优良品系、甘蔗栽培种商业品种以及斑茅克隆。测定了SSR数据的Nei氏遗传距离，并以树状图的形式直观地描绘了各材料之间的关系。在40个甘蔗材料中，观察到遗传距离值在0.60至1.11之间。基因型US-804和US-130之间的遗传距离最短，为0.60。这两个基因型仅在20对引物产生的10条带中存在差异。差异最大的材料是CP-77-400和US-133，遗传距离为1.11。SSR指纹图谱有助于甘蔗育种者厘清商业甘蔗品种的遗传谱系，并评估育种方法的效率。
