Czerniak B, Herz F, Gorczyca W, Koss L G
Department of Pathology, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York 10467.
Cancer. 1989 Oct 1;64(7):1467-73. doi: 10.1002/1097-0142(19891001)64:7<1467::aid-cncr2820640718>3.0.co;2-n.
The expression of the ras gene product p21 in normal gastric mucosa, early gastric carcinoma of diffuse (gastric) and intestinal types, and in adjacent mucosal abnormalities is reported. The analysis was performed on paraffin sections by an immunohistochemical assay using the mouse monoclonal antibody RAP-5 and the rat monoclonal antibody Y13-259. Expression of ras p21 was assessed by staining intensity and percentage of positively stained cells. In comparison to normal gastric mucosa of non-cancer patients, p21 was overexpressed in nearly all early carcinomas of both types and in the dysplastic and/or metaplastic mucosal alterations accompanying intestinal type of gastric cancer. Increased p21 expression was also observed in the normal-appearing mucosa adjacent to early carcinomas of diffuse type, but not in the morphologically normal gastric epithelium adjacent to the intestinal type. The results of this investigation suggest that ras p21 overexpression may be related to early events of human gastric carcinogenesis. The study supports the notion of different pathways in the development of diffuse (gastric) and intestinal types of gastric carcinomas.
本文报道了ras基因产物p21在正常胃黏膜、弥漫型（胃型）和肠型早期胃癌以及相邻黏膜异常中的表达情况。采用小鼠单克隆抗体RAP-5和大鼠单克隆抗体Y13-259，通过免疫组织化学检测对石蜡切片进行分析。通过染色强度和阳性染色细胞百分比评估ras p21的表达。与非癌患者的正常胃黏膜相比，p21在几乎所有两种类型的早期癌以及肠型胃癌伴发的发育异常和/或化生黏膜改变中均过度表达。在弥漫型早期癌相邻的外观正常黏膜中也观察到p21表达增加，但在肠型癌相邻的形态学正常胃上皮中未观察到。本研究结果提示，ras p21过度表达可能与人胃癌发生的早期事件有关。该研究支持弥漫型（胃型）和肠型胃癌发生途径不同的观点。
