Czerniak B, Herz F, Koss L G, Schlom J
Department of Pathology, Montefiore Medical Center, Bronx, NY 10467.
Cancer. 1987 Nov 15;60(10):2432-6. doi: 10.1002/1097-0142(19871115)60:10<2432::aid-cncr2820601014>3.0.co;2-1.
This study was undertaken to determine whether the expression of ras oncogene product p21 can be used as a tumor cell marker of gastric and colonic carcinoma in brush smears. To detect p21 an immunocytochemical assay with RAP-5 monoclonal antibody was used. Benign epithelial gastric cells obtained from normal gastric mucosa or benign gastric lesions reacted negatively in 12 out of 13 cases. Similarly, benign epithelial colonic cells from normal colon or benign colonic lesions were negative for p21 in nine out of ten cases. Weakly positive reaction, confined to a few cell clusters only, was observed in one smear of a benign gastric ulcer and one smear of chronic ulcerative colitis. All 20 smears from colonic carcinoma and all 20 smears of gastric carcinoma contained cells that stained positively for p21, and the degree of tumor differentiation had no impact on the staining pattern. The results recorded in this study show that the immunocytochemical assay for the ras oncogene product may prove to represent a new tool for the cytodiagnosis of gastric and colonic carcinomas.
本研究旨在确定ras癌基因产物p21的表达是否可用作刷片法检测胃和结肠癌的肿瘤细胞标志物。为检测p21，采用了抗RAP-5单克隆抗体的免疫细胞化学检测法。从正常胃黏膜或良性胃部病变获取的良性胃上皮细胞，13例中有12例呈阴性反应。同样，来自正常结肠或良性结肠病变的良性结肠上皮细胞，10例中有9例p21呈阴性。在1例良性胃溃疡涂片和1例慢性溃疡性结肠炎涂片中观察到弱阳性反应，且仅局限于少数细胞簇。所有20例结肠癌涂片和所有20例胃癌涂片均含有p21染色阳性的细胞，肿瘤分化程度对染色模式无影响。本研究记录的结果表明，ras癌基因产物的免疫细胞化学检测可能是胃和结肠癌细胞诊断的一种新工具。
