通过测定腺苷酸环化酶活性检测百日咳博德特氏菌。

Detection of Bordetella pertussis by determination of adenylate cyclase activity.

作者信息

Wirsing von Koenig C H, Tacken A, Hewlett E L

机构信息

Institute for Hygiene and Laboratory Medicine, Municipal Hospital, Krefeld, FRG.

出版信息

Eur J Clin Microbiol Infect Dis. 1989 Jul;8(7):633-6. doi: 10.1007/BF01968146.

DOI:10.1007/BF01968146

PMID:2550232

Abstract

The adenylate cyclase activity of Bordetella pertussis in clinical isolates was measured in calmodulin-supplemented Stainer-Scholte broth by the rate of conversion of ATP to cyclic AMP. Analysis of 250 stock strains of Bordetella pertussis showed that measurable adenylate cyclase activity was produced by all strains. In clinical tests Bordetella pertussis was isolated from 135 (22%) of 605 swab samples. Increased adenylate cyclase activity was detected in 124 (92%) Stainer-Scholte broth cultures of these samples. A total of 475 swabs contained other bacteria or had no growth; only one of the Stainer-Scholte broth cultures of these swab samples contained measurable adenylate cyclase activity. The results indicate that testing for adenylate cyclase activity provides a specific and sensitive means for detecting Bordetella pertussis in clinical specimens.

摘要

采用补充了钙调蛋白的斯坦纳-斯科尔特肉汤，通过ATP转化为环磷酸腺苷的速率来测定临床分离株中百日咳博德特氏菌的腺苷酸环化酶活性。对250株百日咳博德特氏菌菌株的分析表明，所有菌株均产生可测量的腺苷酸环化酶活性。在临床检测中，从605份拭子样本中的135份（22%）分离出了百日咳博德特氏菌。在这些样本的124份（92%）斯坦纳-斯科尔特肉汤培养物中检测到腺苷酸环化酶活性增加。共有475份拭子含有其他细菌或未生长；这些拭子样本的斯坦纳-斯科尔特肉汤培养物中只有一份含有可测量的腺苷酸环化酶活性。结果表明，检测腺苷酸环化酶活性为临床标本中百日咳博德特氏菌的检测提供了一种特异且灵敏的方法。