从成年豚鼠心脏分离心肌细胞的实验方面。
Experimental aspects of isolation of myocytes from adult guinea-pig hearts.
作者信息
Zahradník I, Zahradníková A
机构信息
Centre of Physiological Sciences, Slovak Academy of Sciences, Bratislava.
出版信息
Gen Physiol Biophys. 1989 Apr;8(2):119-32.
Ionic channels can now be effectively studied on enzymatically isolated cardiac myocytes by means of the patch clamp technique. Three procedures reported to give consistently high yields of Ca-tolerant myocytes were tested for applicability to calcium channel studies under our laboratory conditions. None of them was found to be suitable for direct use. Therefore, a modified method for isolation of myocytes from adult guinea-pig hearts was developed. Calcium channel currents measured in Ca-tolerant myocytes isolated by this procedure have been presented and problems of myocytes isolation and of patch-clamp measurement discussed.
现在可以通过膜片钳技术在酶分离的心肌细胞上有效地研究离子通道。我们测试了三种据报道能持续获得高产率耐钙心肌细胞的方法，以确定它们在我们实验室条件下用于钙通道研究的适用性。结果发现它们都不适合直接使用。因此，开发了一种从成年豚鼠心脏分离心肌细胞的改良方法。本文展示了用该方法分离的耐钙心肌细胞中测量到的钙通道电流，并讨论了心肌细胞分离和膜片钳测量的问题。
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