嗜热自养甲烷杆菌δH菌株purE基因上游的转录起始和RNA聚合酶结合位点。

Transcription initiation and a RNA polymerase binding site upstream of the purE gene of the archaebacterium Methanobacterium thermoautotrophicum strain delta H.

作者信息

Brown J W, Reeve J N

机构信息

Molecular, Cellular and Developmental Biology Program, Ohio State University, Columbus 43210.

出版信息

FEMS Microbiol Lett. 1989 Jul 15;51(1):131-6. doi: 10.1016/0378-1097(89)90495-3.

DOI:10.1016/0378-1097(89)90495-3

PMID:2550316

Abstract

DNA-dependent RNA-polymerase (RNAP) purified from the thermophilic archaebacterium Methanobacterium thermoautotrophicum strain delta H has been shown to bind specifically to DNA in the intergenic region upstream of the purE gene cloned from this species. The RNAP binding site has been limited to a 41 bp region of DNA which contains the Box A archaebacterial promoter sequence, 5'ATTAAATA. Transcription of the purE gene appears to initiate in vivo at three locations 20-22 bp downstream of the Box A sequence and 27-29 bp upstream of the ATG translation initiation codon of the purE gene.

摘要

从嗜热古细菌嗜热自养甲烷杆菌δH菌株中纯化出的DNA依赖性RNA聚合酶（RNAP）已被证明能特异性结合从该物种克隆的purE基因上游基因间区域的DNA。RNAP结合位点已被限定在一段41bp的DNA区域，该区域包含古细菌启动子序列Box A，即5'ATTAAATA。purE基因的转录似乎在体内于Box A序列下游20 - 22bp以及purE基因ATG翻译起始密码子上游27 - 29bp的三个位置起始。