克隆的嗜热自养甲烷杆菌转录单元在体外被同源RNA聚合酶特异性转录。
Specific transcription of cloned Methanobacterium thermoautotrophicum transcription units by homologous RNA polymerase in vitro.
作者信息
Knaub S, Klein A
机构信息
Department of Biology, Philipps University, Marburg, FRG.
出版信息
Nucleic Acids Res. 1990 Mar 25;18(6):1441-6. doi: 10.1093/nar/18.6.1441.
Abstract
Specific in vitro transcription by partially purified RNA polymerase from Methanobacterium thermoautotrophicum of DNA sequences cloned in between the promoter and terminator regions of the methyl CoM reductase transcription unit of the same organism is described. The 5'-terminus of the product has been mapped. Deletion analyses of the promoter region show the limits of the sequences essential for the promoter function.
摘要
描述了利用来自嗜热自养甲烷杆菌的部分纯化的RNA聚合酶，对克隆于同一生物体甲基辅酶M还原酶转录单元的启动子和终止子区域之间的DNA序列进行特异性体外转录的过程。已确定了产物的5'-末端。对启动子区域的缺失分析显示了启动子功能所必需的序列界限。
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