Ebralidze A, Tulchinsky E, Grigorian M, Afanasyeva A, Senin V, Revazova E, Lukanidin E
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Genes Dev. 1989 Jul;3(7):1086-93. doi: 10.1101/gad.3.7.1086.
The gene mts1, which is expressed specifically in metastatic cells, was isolated by molecular cloning coupled with differential DNA reassociation. Transcription of mts1 was found not only in tumor cells, but also in normal cells; homologous RNA was detected only in spleen, thymus, bone marrow, and blood lymphocytes. DNA sequencing of mts1 revealed an open reading frame containing information for a peptide of 101 amino acids, and the amino acid sequence suggested that the mts1 protein was identical to the previously isolated Ca2+-binding mouse protein (Jackson-Grusby et al. 1987; Goto et al. 1988). Thus, the mts1 protein is a member of the calcium-modulated protein family, and our data indicate that mts1 is involved in regulating the metastatic behavior of tumor cells.
通过分子克隆结合差异DNA重退火技术，分离出了在转移细胞中特异性表达的基因mts1。发现mts1不仅在肿瘤细胞中转录，在正常细胞中也有转录；同源RNA仅在脾脏、胸腺、骨髓和血液淋巴细胞中检测到。mts1的DNA测序揭示了一个开放阅读框，其中包含一个101个氨基酸的肽段信息，氨基酸序列表明mts1蛋白与先前分离的钙结合小鼠蛋白相同（杰克逊-格鲁斯比等人，1987年；后藤等人，1988年）。因此，mts1蛋白是钙调节蛋白家族的成员，我们的数据表明mts1参与调节肿瘤细胞的转移行为。
