Desai S D, Pasupathy K, Chetty K G, Pradhan D S
Indian J Biochem Biophys. 1989 Apr;26(2):87-91.
Effect of dimethyl sulphoxide (DMSO) on mitochondrial biogenesis in regenerating rat liver and cells of Saccharomyces cerevisiae during aerobiosis has been studied by monitoring the cytochrome oxidase activity. A single dose of DMSO (275 mg/100-125 g body wt) to normal rats stimulated cytochrome oxidase activity in liver mitochondria while the same dose to partial hepatectomized rats inhibited the enzyme activity. Administration of low dose of DMSO (92 mg/100-125 g body wt) to partial hepatectomized rats did not alter the enzyme activity. Anaerobic cells of S. cerevisiae on aerobiosis for 2 hr attained cytochrome oxidase activity level on par with aerobic cells. Inclusion of DMSO (275 mg/100 ml) in the growth medium of S. cerevisiae during respiratory adaptation exerted partial inhibitory effect on the formation of cytochrome oxidase at 2 hr period, while the 10-fold concentration inhibited the enzyme formation completely. However, the inhibitory effect of DMSO on enzyme formation was abolished on prolonged growth (18 hr and above), while these doses had no influence on cytochrome oxidase in aerobic cells of S. cerevisiae. The results imply that DMSO may be exerting its effect on the assembly of subunits into active enzyme complex during mitochondrial biogenesis.
通过监测细胞色素氧化酶活性，研究了二甲基亚砜（DMSO）对需氧条件下再生大鼠肝脏和酿酒酵母细胞线粒体生物发生的影响。给正常大鼠单次注射DMSO（275mg/100 - 125g体重）可刺激肝线粒体中的细胞色素氧化酶活性，而给部分肝切除的大鼠注射相同剂量则会抑制该酶的活性。给部分肝切除的大鼠注射低剂量DMSO（92mg/100 - 125g体重）不会改变该酶的活性。酿酒酵母的厌氧细胞在需氧条件下培养2小时后，其细胞色素氧化酶活性水平与需氧细胞相当。在呼吸适应期间，将DMSO（275mg/100ml）加入酿酒酵母的生长培养基中，在2小时内对细胞色素氧化酶的形成产生部分抑制作用，而10倍浓度则完全抑制该酶的形成。然而，延长培养时间（18小时及以上）后，DMSO对酶形成的抑制作用消失，而这些剂量对酿酒酵母需氧细胞中的细胞色素氧化酶没有影响。结果表明，DMSO可能在线粒体生物发生过程中对亚基组装成活性酶复合物发挥作用。
