Nagami M, Yoshida S, Saitoh T, Takeshita M, Ogawa T
Department of Biochemistry, Medical College of Oita, Japan.
Biochem Int. 1988 Oct;17(4):763-71.
Both Km and Vmax values of cytochrome c oxidase for cytochrome c were elevated in oleic acid-incorporated mitochondria, whereas the amount of oleic acid incorporated into submitochondrial particles was smaller than that into mitochondria and the fatty acid had little effect on the enzyme activity. The degree of change in the bulk membrane fluidity was, however, almost the same in mitochondria and submitochondrial particles. Solubilized cytochrome c oxidase was insensitive to the effect of oleic acid. Oleic acid may act as a modifier of the interaction between cytochrome c oxidase and membrane lipids.
在掺入油酸的线粒体中，细胞色素c氧化酶对细胞色素c的米氏常数（Km）和最大反应速度（Vmax）值均升高，而掺入亚线粒体颗粒中的油酸量比掺入线粒体中的少，且该脂肪酸对酶活性影响很小。然而，线粒体和亚线粒体颗粒中膜整体流动性的变化程度几乎相同。溶解的细胞色素c氧化酶对油酸的作用不敏感。油酸可能作为细胞色素c氧化酶与膜脂相互作用的调节剂。
