Bekpinar S, Oner P, Altug T, Eryürek F, Sürmen E, Deniz G
Department of Biochemistry, Istanbul Faculty of Medicine, University of Istanbul, Capa, Turkey.
Int J Vitam Nutr Res. 1989;59(2):127-30.
The influence of eicosapentaenoic acid (EPA) and vitamin E on brain cortex Ca2+ ATPase activity was examined in rabbits receiving cholesterol-rich diets for a period of 45 days. Rabbits were divided as control (A) and cholesterol-fed groups (B, C, and D). Group C received 80 mg of EPA and group D received 100 IU of vitamin E every day in addition to the cholesterol-rich (2%, w/w) diet which was solely given to Group B. Rabbits receiving cholesterol alone had a significant reduction in brain microsomal phospholipid level. Microsomal free cholesterol and polyunsaturated fatty acids (PUFA) were significantly increased in all experimental groups. Cortex microsomal Ca2+ ATPase activity was found to be inhibited in all cholesterol-fed rabbits as compared to controls, but the highest inhibition was seen in rabbits fed cholesterol alone. Additions of EPA or Vitamin E to the cholesterol-rich diets resulted in a recovery of the enzymatic activity. It is concluded that cholesterol feeding without any addition of PUFA or antioxidant agent might cause an inhibition of brain Ca2+ ATPase activity in rabbits, thereby leading to the dysfunction in ion transport and neurotransmitter release.
在接受富含胆固醇饮食45天的兔子中，研究了二十碳五烯酸（EPA）和维生素E对大脑皮质Ca2+ ATP酶活性的影响。兔子被分为对照组（A）和喂食胆固醇组（B、C和D）。除了仅给予B组的富含胆固醇（2%，w/w）的饮食外，C组每天接受80毫克EPA，D组每天接受100国际单位维生素E。单独接受胆固醇的兔子大脑微粒体磷脂水平显著降低。所有实验组的微粒体游离胆固醇和多不饱和脂肪酸（PUFA）均显著增加。与对照组相比，发现所有喂食胆固醇的兔子的皮质微粒体Ca2+ ATP酶活性均受到抑制，但单独喂食胆固醇的兔子中抑制作用最为明显。在富含胆固醇的饮食中添加EPA或维生素E可使酶活性恢复。得出的结论是，不添加任何PUFA或抗氧化剂的胆固醇喂养可能会导致兔子大脑Ca2+ ATP酶活性受到抑制，从而导致离子转运和神经递质释放功能障碍。
