Chan K Y, Beck T W, Mullen P, Dong W K
Department of Ophthalmology, University of Washington School of Medicine, Seattle 98195.
Invest Ophthalmol Vis Sci. 1989 Sep;30(9):2050-5.
This investigation uses an electrical stimulation model of ocular herpes simplex virus (HSV) reactivation to elucidate the relationship between neuronal protein synthesis, axonal transport and electrical stimulation. In this study, healthy adult albino rabbits were implanted with electrodes over the trigeminal ganglion (TG) and stimulated chronically twice a week for 28-34 days (previously shown to induce HSV reactivation in infected rabbits). The TG was visualized surgically and injected with 3H-proline to label newly synthesized protein. After various times, the TG, corneas and ophthalmic nerve were analyzed for labeled proteins. In nonimplanted and implanted rabbits (whether stimulated or not), the rate of fast anterograde axonal transport in the ophthalmic nerve was 347 mm/day and 326 mm/day, respectively. A lag period of 45 min occurred between initiation of protein labeling and onset of axonal transport. Only a small percentage of newly synthesized neuronal proteins were transported in the ophthalmic nerve (1-4% in the proximal 10 mm) or deposited in the cornea (less than 0.5%) after 24 hr. Neither the rate of protein synthesis in the TG nor the rate of axonal transport was changed by electrical stimulation. However, the amount of radioactively labeled proteins undergoing axonal transport in the stimulated group was five times that of the sham control group. Thus, the routing and loading of TG proteins for axonal transport is enhanced in the electrical stimulation model.
本研究采用眼部单纯疱疹病毒（HSV）再激活的电刺激模型，以阐明神经元蛋白质合成、轴突运输与电刺激之间的关系。在本研究中，将健康成年白化兔的三叉神经节（TG）上方植入电极，每周进行两次慢性刺激，持续28 - 34天（先前已证明该刺激可诱导感染兔子的HSV再激活）。通过手术观察TG，并注射3H - 脯氨酸以标记新合成的蛋白质。在不同时间点后，对TG、角膜和眼神经进行标记蛋白分析。在未植入电极和植入电极的兔子中（无论是否接受刺激），眼神经中快速顺行轴突运输的速率分别为347毫米/天和326毫米/天。蛋白质标记开始与轴突运输开始之间存在45分钟的延迟期。24小时后，只有一小部分新合成的神经元蛋白质在眼神经中运输（近端10毫米处为1 - 4%）或沉积在角膜中（小于0.5%）。电刺激既未改变TG中的蛋白质合成速率，也未改变轴突运输速率。然而，刺激组中进行轴突运输的放射性标记蛋白质的量是假手术对照组的五倍。因此，在电刺激模型中，TG蛋白质用于轴突运输的路径选择和负载能力增强。
