Arvidson B
Acta Neuropathol. 1977 Apr 29;38(1):49-52. doi: 10.1007/BF00691276.
Horseradish peroxidase (HRP) was dripped on the scarified left cornea of adult mice. Twenty-four hours later the animals were fixed by vascular perfusion and frozen sections cut from both trigeminal ganglia. After incubation for peroxidase activity labelled nerve cells were restricted to the medial ophthalmic part of the ganglion ipsilateral to HRP administration. If the scarification was omitted no neuronal labelling was observed. This labelling of the neurons is most probably the result from axonal uptake and subsequent retrograde axonal transport of the tracer. The similarity in distribution of peroxidase labelled nerve cells and the first ganglionic lesions occurring after instillation of herpes simplex virus in the cornea is pointed out.
将辣根过氧化物酶（HRP）滴在成年小鼠划破的左角膜上。24小时后，通过血管灌注固定动物，并从双侧三叉神经节切取冰冻切片。孵育以检测过氧化物酶活性后，标记的神经细胞仅限于给予HRP同侧神经节的眼内侧部分。如果省略划破步骤，则未观察到神经元标记。神经元的这种标记很可能是示踪剂轴突摄取及随后逆行轴突运输的结果。文中指出了过氧化物酶标记神经细胞的分布与角膜滴注单纯疱疹病毒后出现的首批神经节病变分布的相似性。
