Bartfeld H, Dham C, Donnenfeld H, Ollar R A, deMasi M T, Kascsak R
Department of Medicine, St. Vincent's Hospital, New York, NY 10011.
Intervirology. 1989;30(4):202-12. doi: 10.1159/000150094.
Circulating immune complexes were isolated by polyethylene glycol precipitation from the sera of patients with amyotrophic lateral sclerosis. Rabbits immunized with circulating immune complexes from 3 of 5 amyotrophic lateral sclerosis patients induced antisera that specifically reacted with enterovirus-infected cells by immunofluorescence and enzyme-linked immunosorbent assay. These antisera were nonneutralizing and did not react with purified virus. In addition, peripheral lymphocytes of amyotrophic lateral sclerosis patients produced lymphokine in response to extracts from enterovirus (Coxsackie B4) infected cells. These results suggest both a humoral (circulating immune complex) and a cellular immune response in some patients with amyotrophic lateral sclerosis to enterovirus-coded or -induced antigen.
通过聚乙二醇沉淀法从肌萎缩侧索硬化症患者的血清中分离出循环免疫复合物。用5例肌萎缩侧索硬化症患者中3例的循环免疫复合物免疫的兔子产生了抗血清，通过免疫荧光和酶联免疫吸附测定法，这些抗血清能与肠道病毒感染的细胞发生特异性反应。这些抗血清无中和作用，且不与纯化病毒发生反应。此外，肌萎缩侧索硬化症患者的外周淋巴细胞对肠道病毒（柯萨奇B4）感染细胞的提取物产生淋巴因子反应。这些结果表明，在一些肌萎缩侧索硬化症患者中，对肠道病毒编码或诱导的抗原存在体液免疫（循环免疫复合物）和细胞免疫反应。
