Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore, 117585 (Singapore).
Angew Chem Int Ed Engl. 2015 Feb 2;54(6):1780-6. doi: 10.1002/anie.201408476. Epub 2014 Dec 11.
Activatable photosensitizers (PSs) have been widely used for the simultaneous fluorescence imaging and photodynamic ablation of cancer cells. However, the ready aggregation of traditional PSs in aqueous media can lead to fluorescence quenching as well as reduced phototoxicity even in the activated form. We have developed a series of PSs that show aggregation-enhanced emission and phototoxicity and thus the exact opposite behavior to that of previously reported PSs. We further developed a dual-targeted enzyme-activatable bioprobe based on the optimized photosensitizer and describe simultaneous light-up fluorescence imaging and activated photodynamic therapy for specific cancer cells. The design of smart probes should thus open new opportunities for targeted and image-guided photodynamic therapy.
可激活的光敏剂 (PSs) 已被广泛用于癌细胞的荧光成像和光动力消融的同步检测。然而，传统 PSs 在水介质中容易聚集，导致荧光猝灭，即使在激活状态下也会降低光毒性。我们开发了一系列具有聚集增强发光和光毒性的 PSs，其行为与之前报道的 PSs 完全相反。我们进一步基于优化的光敏剂开发了一种双靶酶激活的生物探针，并描述了针对特定癌细胞的同步点亮荧光成像和激活光动力治疗。因此，智能探针的设计为靶向和图像引导的光动力治疗开辟了新的机会。
