Belal F, Sharaf El-Din M, Tolba M M, Alaa H
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516, Mansoura, Egypt.
Luminescence. 2015 Sep;30(6):805-11. doi: 10.1002/bio.2823. Epub 2014 Dec 11.
A highly sensitive, simple and rapid spectrofluorimetric method was developed for the determination of lacidipine (LCP) in tablets. The proposed method is based on the investigation of the fluorescence spectral behavior of LCP in both sodium dodecyl sulphate (SDS) and the tween-80 micellar system. In aqueous solutions of acetate buffer (pH 4.5), the fluorescence intensities of LCP were greatly enhanced (ca. 2.4 and 4.3 folds) in the presence of either SDS or tween-80, respectively. The fluorescence intensity was measured at 444 nm after excitation at 277 nm using either SDS or tween-80 as a surfactant. The fluorescence-concentration plots were rectilinear over the ranges of 50.0-500.0 ng/ml and 5.0-200.0 ng/ml with lower detection limits of 5.11 and 2.06 ng/ml and lower quantification limits of 17 and 6.87 ng/ml using SDS and tween-80, respectively. The method was successfully applied to the analysis of LCP in commercial tablets and the results were in good agreement with those obtained with the comparison method. Furthermore, content uniformity testing of pharmaceutical tablets was also conducted.
建立了一种高灵敏度、简单快速的荧光分光光度法用于测定片剂中的拉西地平（LCP）。该方法基于研究拉西地平在十二烷基硫酸钠（SDS）和吐温-80胶束体系中的荧光光谱行为。在醋酸盐缓冲液（pH 4.5）的水溶液中，分别在SDS或吐温-80存在下，LCP的荧光强度大大增强（约2.4倍和4.3倍）。以SDS或吐温-80作为表面活性剂，在277 nm激发后于444 nm处测量荧光强度。使用SDS和吐温-80时，荧光浓度曲线在50.0 - 500.0 ng/ml和5.0 - 200.0 ng/ml范围内呈线性，检测限分别为5.11和2.06 ng/ml，定量限分别为17和6.87 ng/ml。该方法成功应用于市售片剂中LCP的分析，结果与对照方法所得结果良好吻合。此外，还进行了药物片剂的含量均匀度测试。
