Chen Fangjiao, Su Yue, Zhang Fang, Guo Yinlong
Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, Shanghai, China; National Center for Organic Mass Spectrometry in Shanghai, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, China.
J Sep Sci. 2015 Feb;38(4):670-6. doi: 10.1002/jssc.201400862. Epub 2015 Jan 16.
The total saccharides content of Lycium barbarum L. is very high, and a high temperature would result in saccharide decomposition and the emergence of a large amount of water. Moreover, the volatile compounds from the fruit of L. barbarum L. are rather low in concentration. Hence, it is difficult for a conventional headspace method to study the volatile compounds from the fruit of L. barbarum L. Since headspace-trap gas chromatography with mass spectrometry is an excellent method for trace analysis, a headspace-trap gas chromatography with mass spectrometry method based on low-temperature (30°C) enrichment and multiple headspace extraction was developed to explore the volatile compounds from the fruit of L. barbarum L. The headspace of the sample was extracted in 17 cycles at 30°C. Each time, the compounds extracted were concentrated in the trap (Tenax TA and Tenax GR, 1:1). Finally, all the volatile compounds were delivered into the gas chromatograph after thermal desorption. With the method described above, a total of 57 compounds were identified. The identification was completed by mass spectral search, retention index, and accurate mass measurement.
枸杞的总糖含量很高，高温会导致糖类分解并产生大量水分。此外，枸杞果实中的挥发性化合物浓度较低。因此，传统的顶空法很难研究枸杞果实中的挥发性化合物。由于顶空捕集气相色谱-质谱联用是一种出色的痕量分析方法，因此开发了一种基于低温（30°C）富集和多次顶空萃取的顶空捕集气相色谱-质谱联用方法，以探索枸杞果实中的挥发性化合物。样品的顶空在30°C下进行17次循环萃取。每次萃取的化合物都在捕集阱（Tenax TA和Tenax GR，1:1）中浓缩。最后，所有挥发性化合物在热解吸后被送入气相色谱仪。采用上述方法，共鉴定出57种化合物。通过质谱检索、保留指数和精确质量测量完成了鉴定。
