Leone G, Volpato M D, Nelli N, Lamponi S, Boanini E, Bigi A, Magnani A
Department of Biotechnology, Chemistry and Pharmacy, University of Siena (INSTM), via Aldo Moro 2, Siena, 53100, Italy.
Via Fratelli Rosselli, 16-Colle Di Val D'elsa, Siena, Italy.
J Biomed Mater Res A. 2015 Aug;103(8):2521-30. doi: 10.1002/jbm.a.35389. Epub 2014 Dec 19.
Cartilage is a highly organized avascular soft tissue that assembles from nano-to macro-scale to produce a complex structural network. To mimic cartilage tissue, we developed a stable multilayered composite material, characterized by a tailored gradient of mechanical properties. The optimized procedure implies chemical crosslinking of each layer directly onto the previous one and ensures a drastic reduction of the material discontinuities and brittleness. The multilayered composite was characterized by infrared spectroscopy, differential scanning calorimetry, thermogravimetry, and scanning electron microscopy in order to compare its physico-chemical characteristics with those of cartilage tissue. The rheological behavior of the multilayered composite was similar to that of human cartilage. Finally its cytocompatibility toward chondrocytes and osteoblasts was evaluated.
软骨是一种高度组织化的无血管软组织，它从纳米尺度到宏观尺度组装形成一个复杂的结构网络。为了模拟软骨组织，我们开发了一种稳定的多层复合材料，其特点是具有定制的机械性能梯度。优化后的工艺意味着将每一层直接化学交联到上一层上，并确保材料的不连续性和脆性大幅降低。通过红外光谱、差示扫描量热法、热重分析法和扫描电子显微镜对多层复合材料进行了表征，以便将其物理化学特性与软骨组织的特性进行比较。多层复合材料的流变行为与人体软骨相似。最后评估了其对软骨细胞和成骨细胞的细胞相容性。
