地衣形成真菌石果梅衣（梅衣科）中真菌特异性微卫星标记的表征
Characterization of fungus-specific microsatellite markers in the lichen-forming fungus Parmelina carporrhizans (Parmeliaceae).
作者信息
Alors David, Dal Grande Francesco, Schmitt Imke, Kraichak Ekaphan, Lumbsch H Thorsten, Crespo Ana, Divakar Pradeep K
机构信息
Departamento de Biología Vegetal II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza de Ramón y Cajal s/n, Madrid 28040, Spain.
Biodiversity and Climate Research Centre (BiK-F), Senckenberg Gesellschaft für Naturforschung, 60325 Frankfurt am Main, Germany.
出版信息
Appl Plant Sci. 2014 Nov 20;2(12). doi: 10.3732/apps.1400081. eCollection 2014 Dec.
PREMISE OF THE STUDY
Microsatellite loci were developed to study the lichen-forming fungus Parmelina (Parmeliaceae) in different habitats of western Europe and the Mediterranean for baseline studies to understand the effects of climate change on its distribution. •
METHODS AND RESULTS
We cultured P. carporrhizans from ascospores for genomic sequencing with Illumina HiSeq. We successfully developed 11 polymorphic microsatellite markers and associated primer sets and assessed them with 30 individuals from two of the Canary Islands. The average number of alleles per locus was 8.8. Nei's unbiased gene diversity of these loci ranged from 0.53 to 0.91 in the tested populations. Amplification in two closely related species (P. tiliacea, P. cryptotiliacea) yielded only limited success. •
CONCLUSIONS
The new microsatellite markers will allow the study of genetic diversity and population structure in P. carporrhizans. We propose eight markers to combine in two multiplex reactions for further studies on a larger set of populations.
研究前提
开发微卫星位点以研究地衣形成真菌帕氏地卷（Parmeliaceae）在西欧和地中海不同栖息地的情况，作为基线研究，以了解气候变化对其分布的影响。
方法与结果
我们从子囊孢子培养了根生帕氏地卷用于Illumina HiSeq基因组测序。我们成功开发了11个多态性微卫星标记及相关引物组，并用来自加那利群岛两个岛屿的30个个体对其进行了评估。每个位点的平均等位基因数为8.8。在测试群体中，这些位点的内氏无偏基因多样性范围为0.53至0.91。在两个近缘物种（阔叶帕氏地卷、隐卷帕氏地卷）中的扩增仅取得有限成功。
结论
新的微卫星标记将有助于研究根生帕氏地卷的遗传多样性和种群结构。我们建议在两个多重反应中组合使用八个标记，以便对更多群体进行进一步研究。
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