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研究肺衣（Lobariaceae）中地衣真菌肺衣（Lobaria pulmonaria）微卫星位点的特征。

Characterization of microsatellite loci in the lichen fungus Lobaria pulmonaria (Lobariaceae).

机构信息

Swiss Federal Research Institute WSL, Zuercherstrasse 111, CH-8903 Birmensdorf, Switzerland.

出版信息

Appl Plant Sci. 2013 Jan 31;1(2). doi: 10.3732/apps.1200290. eCollection 2013 Feb.

DOI:10.3732/apps.1200290
PMID:25202516
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4105370/
Abstract

PREMISE OF THE STUDY

Microsatellite loci were developed for the threatened haploid lichen fungus Lobaria pulmonaria to increase the resolution to identify clonal individuals, and to study its population subdivision. •

METHODS AND RESULTS

We developed 14 microsatellite markers from 454 DNA sequencing data of L. pulmonaria and tested for cross-amplification with L. immixta and L. macaronesica. The number of alleles per locus ranged from two to 23. Nei's unbiased gene diversity, averaged over loci, ranged from 0.434 to 0.517 in the three studied populations. •

CONCLUSIONS

The new markers will increase the genetic resolution in studies that aim at disentangling clones in L. pulmonaria and may be useful for closely related species within Lobaria sect. Lobaria.

摘要

研究前提

为受威胁的单倍体地衣真菌肺衣开发微卫星基因座，以提高识别克隆个体的分辨率，并研究其种群划分。

方法和结果

我们从肺衣的 454 DNA 测序数据中开发了 14 个微卫星标记，并测试了它们与肺衣和马卡罗尼西亚肺衣的交叉扩增。每个基因座的等位基因数从 2 到 23 不等。在三个研究种群中，平均每个基因座的Nei 无偏基因多样性从 0.434 到 0.517 不等。

结论

新标记将提高旨在分离肺衣中克隆的遗传分辨率，并且可能对 Lobaria 组内的密切相关的物种有用。

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